If you have a compound that elutes from an LC column at 8.2 mins, what are the mobile phase conditions at that time? Mobile phase ramp: t0-25:75 (AcN:H2O); isocratic until 5 min; 5 min to 10 min, linear ramp up to 100% AcN; hold at 100% ACN for 5 min; re-equilibrate at 25:75 for 2 min.
If you have a compound that elutes from an LC column at 8.2 mins, what are the mobile phase conditions at that time? Mobile phase ramp: t0-25:75 (AcN:H2O); isocratic until 5 min; 5 min to 10 min, linear ramp up to 100% AcN; hold at 100% ACN for 5 min; re-equilibrate at 25:75 for 2 min.
Chapter91: Gas Chromatography
Section: Chapter Questions
Problem 1P
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If you have a compound that elutes from an LC column at 8.2 mins, what are the mobile phase conditions at that time? Mobile phase ramp: t0-25:75 (AcN:H2O); isocratic until 5 min; 5 min to 10 min, linear ramp up to 100% AcN; hold at 100% ACN for 5 min; re-equilibrate at 25:75 for 2 min. *
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