13. Consider the TLC plate below to answer the question. What is the Rf of the top component? Note: the distance between the origin and the bottom spot is 1.4 cm. The distance between the two spots is 1.6 cm. Solvent front 7.2 8.4 1.6 Baseline
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- Different from GCMS, why is an interface needed when coupling HPLC with MS?Silica gel is very polar stationary phase. Therefore, more polar molecule sticks to silica gel on the TLC plate and has higherRfvalue. True FalseAn ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC developing conditions to obtain an RF of 0.30 if it's too low or too high (Hint: Think about chromatography phases).
- It is never necessary to take more than one composite sample per field. True Or FalseWhen the use of 2D Thin layer Chromatography is better than normal TLC? Please shortly answer at your own words. Answer should be to the point (specific 4-5 lines).The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
- What is the result of applying too much sample to the TLC plate? Select one: Some of the sample moves down instead of up Reduced separation between the spots O The sample moves past the solvent front O The spot does not leave the origin O The sample leeches out into the elution solventzero absorbance or 100 %T. If not, adjust the spectrophotometer accordingly. (2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5) in a cuvet following the "blank solution procedure" as above. Measure and record the wavelength and absorbance. Scan the wavelength range of the spectrophotometer while recording absorbance values. You will need to scan manually, setting the absorbance at zero (100% T) with the blank solution (0.1 M HNO3) at EACH chosen wavelength before recording the absorbance of the stock solution. Plot the data, absorbance, A (y-axis) versus the wavelength, λ (x-axis), to determine the λmax, the wavelength where maximum absorbance occurs for the cobalt ion. Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 300 400 500. 600 Wavelength (nm) A representative absorption spectrum of a laboratory solution. 148 Page .700 (1) Absorbance of the Standard Solutions. 800 4 of 9 O - ZOOM +If the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoning
- What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography (TLC)? Please shortly answer at your own words. Answer should be to the point ( specific 4-5 lines).5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.Two components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 min and the peak widths are approximately equal. Calculate the minimum number of theoretical plates needed to achieve an acceptable resolution
