E14. A sample of DNA was subjected to automated DNA sequencing and the output is shown here. WWww MWWWwW.INY G = Black A = Green T = Red C= Blue What is the sequence of this DNA segment?
Q: Transformation can be described as Polymerase chain reaction Sanger Sequencing Use of…
A: DNA is one of the genetic material present in the cell. The cell is modified by inserting the…
Q: purpose(s) of DNA extraction
A: The purpose of DNA extraction are: To study the genetic cause of the disease. For the development…
Q: How many subunits does the E. coli DNA polymerase I have? Select one: O a. 5 Оb. 10 О с. 1 O d. 3
A: RNA polymerase is required for the transcription of DNA into RNA. It is also important in the study…
Q: The enzyme that proofreads the new strand of DNA, looking for and correcting mistakes as it…
A: DNA replication is the process in which the two identical replicas of DNA from one original DNA…
Q: The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b.…
A: Restriction enzymes:- these are the proteins and mostly isolated from prokaryotes. These enzymes…
Q: Which DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides?…
A: DNA polymerase is the enzyme that catalyses the replication of DNA. DNA polymerase cannot synthesise…
Q: DNA gyrasea. promotes negative supercoiling.b. relaxes positive supercoils.c. cuts DNA strands as…
A: Deoxyribonucleic acid (DNA) replication is the biological process of producing two identical…
Q: utline the four broad classes of chemical damage to DNA.
A: Epigenetic mechanisms are described in detail. Multiple biological processes that affect epigenetic…
Q: Describe the basic features of recombinant DNA, the polymerase chain reaction, and DNA fi…
A: Introduction: The direct modification of an organism's genes using biotechnology is known as genetic…
Q: Which of the following statements is accurate for DNA replication in your cells, but not PCR? a.…
A: BASIC INFORMATION ABOUT GENETIC MATERIAL DNA It stands for deoxyribo nucleic acid. It is the…
Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…
A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…
Q: From among only the following, the THIRD step in DNA replication is Primase produces an RNA primer…
A: Primase creates an RNA primer, which is a brief stretch of complementary nucleic acid that serves as…
Q: DNA polymerase III is a processive enzyme, which means that a. it does not dissociate from the…
A: Ribonudeic acid is produced by an enzyme called RNA polymerase, which is responsible for the…
Q: h of the following is NOT true of cutting DNA in biotechnology? A molecular biologist can cut DNA…
A: Biotechnology is a technique in which DNA is manipulated either by cutting the the and required gene…
Q: What to restriction enzymes do? O They cut at specific locations on both strands in the…
A:
Q: library contains a collection of DNA clones derived from MRNAS. library contains a collection of DNA…
A: Genomic library is a library which encompasses the entire genome. Genomic Library involves the…
Q: DNA replication is said to be semiconservative because a. one of the new molecules conserves both of…
A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…
Q: Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in: A) Recombinant…
A: VNTRs are repeated units that occur in the genome and display length variations. The VNTRs consist…
Q: DNA cloning isa. making multiple genetically identical cells.b. making multiple copies of a piece of…
A: Molecular biology deals with the molecular basis of the cells including the synthesis,…
Q: The purpose of using a washing soap in DNA extraction experiment is to O a. Lyse the cells to…
A: DNA extractions is a method to isolate DNA from the nucleus of given cell culture. The four steps of…
Q: 1. This image summarizes how computers sort and order DNA fragments to produce a final sequence of…
A: Nucleic acid is chemically different than protein and carbohydrate . This difference helps in…
Q: Recombinant DNA is:(a) DNA that is produced when genes from one kind oforganism are introduced by…
A: A gene is an essential unit of heredity and a sequence of nucleotides in DNA or RNA that encodes the…
Q: 5. Below is an image of DNA sequenced using the Sanger sequencing method, where different…
A: Gel electrophoresis is a molecular technique that aims to separate the DNA, RNA, and protein…
Q: The enzyme ________ separates the TWO DNA strands of the double helix. a. helicase b. ligase…
A: Deoxyribonucleic acid (DNA) is the functional genetic information-carrying molecule present in cells…
Q: DNA Polymerase holoenzymes used for DNA replication recognizes A. double-stranded sequences as…
A: DNA polymerase III holoenzyme (Pol III HE) is an enzyme that catalyzes elongation of DNA chains…
Q: Once separated from other cellular components, extracted DNA can be used for a variety of purposes.…
A: Introduction:- Biotechnology relies heavily on the extraction of DNA. It serves as the foundation…
Q: Which of the following best explains the production of Okazaki fragments in replicating DNA (a) DNA…
A: Okazaki fragments also known as lagging strand in DNA replication is a strand whose RNA primers are…
Q: In enzymes cut DNA into fragments, which are separated by size to form a pattern of bands. O…
A: In DNA fingerprinting , restriction enzyme are used to cut DNA into smaller fragments. Then these…
Q: The bacterial repair system that corrects mismatched bases after polymerization is able to…
A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…
Q: What does a restriction enzyme do? O a) Delivers recombinant DNA to target cells. O b) Cuts DNA at a…
A: Recombinant DNA technology is the emerging branch in biotechnology which has a great potential. It…
Q: Ultraviolet light principally causes which of the following dmages to DNA? A methylation of specific…
A: UV light can kill cells by damaging DNA to an irreversible extent. The light initiates reaction…
Q: fa restriction endonuclease recognizes and cleaves a linear piece of DNA and Circular DNA at 8…
A: Restriction endonuclease These are also known as restriction enzymes. These enzymes have been…
Q: Describe the activities of the enzymes required to construct a recombinant DNA molecule.
A: Genetic engineering is the major area for the production of recombinant DNA. The recombinant DNA is…
Q: What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGCCCGTATAC -5…
A: DNA stands for deoxyribonucleic Acid. It is a long polymer of deoxyribonucleotides. It is found in…
Q: he purpose of using a washing soap in DNA extraction experiment is to D a. Cut the proteins away…
A: DNA stands for deoxyribonucleic acid. It is present in the nucleus of the cell It stores genetic…
Q: 1. The polymerase chain reaction (PCR) is used by scientists to amplify DNA, particularly when the…
A: We are answering 1st question only. For the rest of the questions please repost. The polymerase…
Q: A linear piece of DNA is cut as follows: cut with Ecorl - get 1kb and 4 kb band Cut with Bamhl - get…
A: Ans- According to question For linear DNA – Upon Cutting by for Eco R1 it formed – 1kb and 4kb =…
Q: The leading strand in DNA replication requires only one primer for its synthesis. The lagging strand…
A: DNA replication is the semi-conservative process in which each strand of DNA molecule act as a…
Q: Which of the following items do not affect the melting temperature of DNA? A length of the DNA B the…
A: Melting temperature ( Tm): half of DNA gets denatured (melts). Tm = 2* (A+T) + 4* (G+C)
Q: What is a DNA Ladder? a solution added to a DNA sample to give it color for an electron microscope.…
A: DNA: The hereditary substance in humans and almost all other animals is DNA, or deoxyribonucleic…
Q: The DNA molecule is replicating. Below are four DNA fragments. DNA Fragments: 1. CGATCT 2. TCACGA 3.…
A: The Nucleotide polymers that are responsible for determining and regulating the genetic…
Q: The direct manipulation of genes is called O genetic engineering O DNA sequencing nucleic acid…
A: Gene can be defined as a unit of heredity.It is a segment of DNA(nucleotide sequence)that may…
Q: Which of the following is/are not required for DNAreplication to occur?a. DNA polymerase d.…
A: DNA (short for deoxyribonucleic acid) is a double helical structure (with two DNA strands). A…
Q: 1) What is the size of the following single-stranded piece of DNA? ATCGTGTGCT A) 10 b B) 20 bp C) 20…
A: According to guidelines we have to answer the first question only. so please kindly post the…
Q: The extraction of DNA is a common procedure in biotechnology research laboratories. Research the…
A: Common DNA extraction methods Different extraction methods result in different yields and purity of…
Q: Which of the following is not required for Sanger sequencing of DNA? OA. ddNTPs B. DNTPS C. A DNA…
A: Sanger sequencing is a method of DNA sequencing.It involves selective incorporation of chain…
Q: DNA A= 5' GGG GCT AGC CCC 3' DNA B=3' ATA TAT ATA CCC 5' DNA C= 5' TAC GTT ACG TCG 3' DNA D= 3' ATC…
A: A hairpin loop has a stem that contains complementary bases and a circular structure that does not…
Q: Use the set of gene sequencing results below to answer the question that follows: A G C T -Wells I…
A: Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins…
Q: DNA polymerase III requires a(n)__________ for synthesis of DNA to occur A- DNA template B-RNA…
A: DNA polymerase III binds with the primers on the leading and lagging strand and synthesize new DNA…
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- Write out the resulting DNA molecules after the following double stranded DNA molecule is digested with EcoRI: 5’-ATGTTAGAATTCTACGTCGAATTCAAATTT-3’ 3’-TACAATCTTAAGATGCAGCTTAAGTTTAAA-5’1. The extraction of DNA is a common procedure in biotechnology research laboratories. Research the methods of extracting DNA in biotechnology facilities. Describe how the extraction methods used in this lesson compare to those of research laboratories.What implication does eating DNA have on the commercial properties of DNA in your hair conditioner, nutritional supplements, skin cream, or genetically modified plants and animals?
- 5. Transformation can be described as Polymerase chain reaction Sanger Sequencing Use of restriction enzyme to cut DNA Pieces of DNA are ligated Insertion of foreign DNA into bacteriaC. Why does isolated DNA appear stringy?Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.
- Describe the characteristics of the extracted DNA, such as colour, shape, size, and consistency.Examine the DNA model, what are the features that contribute to the stability and the ability of the DNA to replicate faithfully? Identify the specific molecule represented by the following colored plastic chips. Color Molecule Represented BrownWhiteOrangeBlueGreenRedPinkYellowD. Using the DNA sequence above, write a new DNA sequence from 3’ to 5’ that incorporates a transversion leading to a missense mutation in the third amino acid. Bold or underline the nucleotide that has been changed and indicate the new amino acid
- 1. What is/are the purpose(s) of DNA extraction? 2. What is the function of each reagent used in the extraction of DNA from a banana? a. Saltwater b. Liquid dishwashing soap c. Cold isopropyl alcoholB. Using the DNA sequence above, write a new DNA sequence from 3’ to 5’ that incorporates a transition leading to a silent mutation in the second amino acid. Bold or underline the nucleotide that has been changed1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)