The leading strand in DNA replication requires only one primer for its synthesis. The lagging strand requires several primers because a. the lagging strand is prepared in short segments b. the lagging strand lacks a topoisomerase c. the leading strand serves as a template for the lagging strand d. All DNA polymerases read from the 5′ end.

The leading strand in DNA replication requires only one primer for its synthesis. The lagging strand requires several primers because a. the lagging strand is prepared in short segments b. the lagging strand lacks a topoisomerase c. the leading strand serves as a template for the lagging strand d. All DNA polymerases read from the 5′ end.

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Description: The leading strand in DNA replication requires only one primer for its synthesis. The lagging strand requires several primers because a. the lagging strand is prepared in short segments b. the lagging strand lacks a topoisomerase c. the leading stran

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter8: Dna Structure And Function

Section: Chapter Questions

Problem 9SA: Energy that drives the attachment of a nucleotide to the end of a growing strand of DNA comes from...

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The initial mechanism for repairing nucleotide errors in DNA is _____. a. mismatch repair b. DNA polymerase proofreading c. nucleotide excision repair d. thymine dimers
DNA replication requires ________ . a. DNA polymerase c. primers b. nucleotides d. all are required
In the dideoxy-sequencing reaction, what terminates DNA synthesis at a particular base? a. The absence of a base on the ddNTP halts the DNA polymerase. b. The ddNTP causes a break in the sugar–phosphate backbone. c. DNA polymerase will not incorporate a ddNTP into the growing DNA strand. d. The absence of a 3′-OH group on the ddNTP prevents the addition of another nucleotide.
DNA polymerases ____. a. add new nucleotides to a strand b. repair DNA c. assemble new strands in both direction d. seal gaps in the sugar-phosphate backbone e. catalyze carbon bonding
DNA strands are anti-parallel and DNA polymerase can only synthesize DNA in a 5' to 3' direction. How does the enzyme synthesize both strands at the same time? A. The leading strand is sythesised in Okazaki fragments B. The lagging strand is synthesised in short Okazaki fragments. C. Only one strand is replicated. D. There are more than one DNA polymerase involved.
In DNA replication, the role of topoisomerase is to Question 11 options: a) "unzip" the double stranded DNA in front of DNA polymerase. b) maintain the single stranded DNA. c) supercoil the DNA after the replication fork has passed. d) relieve supercoil tension in the DNA in front of the replication fork.
When a dideoxyribonucleotide is incorporated into a growingDNA strand,a. the strand elongates faster.b. the strand cannot elongate.c. the strand becomes more susceptible to DNase I cleavage.d. none of the above occurs.
In DNA replication, a primer is _____. a. what the original DNA strands are called b. a molecule that provides the energy for nucleotide attachments c. a regulatory protein that turns on the gene that starts DNA replication d. an enzyme that breaks the hydrogen bonds between base pairs e. a short piece of nucleic acid that serves as an attachment point for DNA polymerase
DNA polymerase adds nucleotides to Select one: a. the 5′ end, and the leading strand elongates in the 3′ to 5′ direction b. the 3′ end, and the leading strand elongates in the 5′ to 3′ direction c. the 5′ end, and the leading strand elongates in the 5′ to 3′ direction d. the 3′ end, and the leading strand elongates in the 3′ to 5′ direction
The problem of synthesizing the lagging strand, in the sense that DNA synthesis can only occur in one direction, is manifested inA. creation of primers every so often as more of the template strand isuntwisted.B. formation of shorter DNA strands called Okazaki fragments.C. the need for the enzyme ligase to link short DNA strands together.D. All of the above choices are correct.
DNA replication is said to be semiconservative because a. one of the new molecules conserves both of the original DNA strands. b. the new DNA molecule contains two new DNA strands. c. both of the new molecules contain one new strand and one old strand. d. DNA polymerase conserves both of the old strands.
Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.