Why is it important to acquire a reference spectrum and subtract it from the sample’s spectrum? Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)?What would happen if you put too much of a compound in the cuvette during the spectral measurement? Why is it important to avoid any scratch on the cuvette? Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not?

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Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.17QAP: Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC...

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Why is it important to acquire a reference spectrum and subtract it from the sample’s spectrum?
Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)?
What would happen if you put too much of a compound in the cuvette during the spectral measurement?
Why is it important to avoid any scratch on the cuvette?
Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not?

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