Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: Principle of the test behind Supravital staining for Heinz bodies
A: Heinz bodies are development within red blood cells collected of contaminated hemoglobin. They are…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: List other fluorescent stains. What is the usefulness of each?
A: Fluorescent stains/dyes are non-protein molecules that are employed in visualizing samples under a…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…
A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…
Q: what are the regeants and results for Mann’s methyl blue-eosin stain
A: Mann's methyl blue eosin strain- is formulated to demonstrate Negri bodies in the tissue section it…
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
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A: It is a contagious virus which is known for causing diarrhea. It is present in infected persons…
Q: Defi ne dyes and describe the basic chemistry behind the process of staining.
A: Staining is a method by which contrast in a sample is enhanced usually at the microscopic level.…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: Notes on Mann's methyl -blue eosin stain
A: Staining is a technique that is used in microscopy to visualize the cells and cell components in a…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…
A: Spectrophotometric analysis has been widely used in biomedical science to determine the…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…
A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…
Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…
A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…
Q: Discuss sabin feldman dye test.
A: A Sabin–Feldman dye test is a serologic test for toxoplasmosis diagnosis. Toxoplasma trophozoites…
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: What's the theory and conclusion of a acid fast stain?
A: It was Ziehl who originally devised differential staining procedures, that were later refined by…
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: Protocol for Mann's methyl blue eosin stain technique
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Q: Step-by-step smear preparation of Grams stain
A: Gram staining is a laboratory technique that is used to culture bacteria and identifies two kinds of…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: What is safe view dye for? Compare it with Ethidium Bromide.
A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: Give the color reaction in Wright’s stain and the function of these
A: Wright's stain is a polychromatic stain consisting of a mixture of eosin and methylene Blue. It is a…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: Why don't you heatfix a negative stain slide?
A: Negative staining is used to investigate the physical structure, size, and organization of…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: What is the importance of sllid width in fluorescence spectroscopy
A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.
Q: list the stains and decolorizing agent used in acid fast staining method.
A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…
Q: Give two factors that may affect a good gram staining technique output. Provide an explanation how…
A: Many factors can affect the results of gram staining. However, two important factors that may affect…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin…
A: Polychromatic solution of Methyl Blue and Eosin Y stains (modified Mann's solution) is used for…
Q: What is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).
A: Introduction: It is the most powerful tool to study the interactions between matter and…
Q: Write down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
A: It is a differential staining technique which was first developed by Ziehl and later on modified by…
Q: What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic…
A: Simple staining: * The process staining the bacterial cell with a dye to see bacterial structure…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: Briefly describe the uses of Special stains.
A: Routine stain: It refers to the hematoxylin and eosin (H&E) staining that is used routinely to…
Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…
A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…
Q: Congo red is a red acidic stain and methylene blue is a l a bacterial smear with a mixture of eosin…
A: Eosin Methylene Blue (EMB) agar is both selective and differential culture medium. It is a selective…
Principle and procedure for Mann’s methyl blue-eosin stain
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- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninWhen it comes to simple stains, why is time so important? And what is the purpose of rinsing the slide in water after each Gram staining procedure?Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.