Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: Why safranin is used as the sample dye?
A: Safranin is utilized as a counterstain in some staining conventions, shading cell cores red. This is…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Principle and procedure for Mann’s methyl blue-eosin stain
A: Methyl blue stain Methylthioninium chloride or methyl blue is a stain use to highlight animal…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: What are other staining methods that can be used for PAGE?
A: Introduction: PAGE electrophoresis is a subtype of gel electrophoresis whereby normal gel is…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: What does the red dye Prontosil have to do with sulfa drugs?
A: Antibiotics are the medicines which are used to cure infections which are caused by bacteria. These…
Q: What is G-banding? O A) Most commonly used banding method B) Uses giemsa dye O C) Produces…
A: A chromosome contains the genetic material, it is present in the nucleus in condensed form.
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…
A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: Molecules are tagged by antibodies in __________ microscopy. a. Confocal b. Differential…
A: Microscopy is defined as that technical field that makes use of microscopes in order to visualize…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: What are the functions of a primary stain and a counterstain?
A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: explain the spotting effect of ethyl alcohol, acetone and coconut oil when place into the paper
A: When ethyl alcohol is spotted onto paper, it wets the paper and after some time this spot will…
Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…
A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: Protocol for Mann's methyl blue eosin stain technique
A: The anterior pituitary gland and viral appearance are stained with a polychromatic solution of…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…
A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: Why don't you heatfix a negative stain slide?
A: Negative staining is used to investigate the physical structure, size, and organization of…
Q: Select all of the following that represent examples of positive stains. Check All That Apply…
A: Staining is the common laboratory procedure which can be specific and general according to the use.…
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: list the stains and decolorizing agent used in acid fast staining method.
A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…
Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?
A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic…
A: Simple staining: * The process staining the bacterial cell with a dye to see bacterial structure…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain…
A: Cationic Dyes- These are the dyes which dissociates into cations in aqueous solutions. Anionic Dyes-…
Q: Name two fixatives used in staining?
A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
Step by step
Solved in 2 steps
- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninSelect all of the following that represent examples of positive stains. A) nigrosin B) methylene blue C) crystal violet D) India ink E) safraninIn staining, why is there a need for contrasting stains?
- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.What is the difference between Giemsa and Wright stain?
- What is the difference between simple and differential stains? Give an example of a differential stain.Is it possible to perform double or triple staining using fluorescent dyes? Explain.What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?