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A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Describe the Gram staining procedure
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Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
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Q: What is the principle application of negative staining?
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Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
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Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
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Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
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Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: What is the difference between simple stains anddifferential stains?
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Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
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Q: What are the functions of a primary stain and a counterstain?
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Q: a. Why must heat be used with the application of the primary stain during endospore staining?
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Q: Which step of the gram stain procedure is most critical?why?
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Q: Differentiate the “hot method” and “cold method” of acid-fast staining
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Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
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Q: list the stains and decolorizing agent used in acid fast staining method.
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Q: What is the best streaking technique in a culture media? Explain
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Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…
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Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?
A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: For What purpose is Giemsa stain used for?
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Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
Q: How can the thickness of the smear affect the staining procedure?
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Q: Name two fixatives used in staining?
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What is the secondary stain in both acid-fast stain methods?
The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it contains the rigid cell wall. It is classified into different types based on the structure namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged and pleomorphic bacteria
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- What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary stain in the Ziehl-Neelsen method?
