For scanning, the wavelength range is selected from 400 nm to 200 nm with medium scanning speed since cefuroxime is well-absorbed at an emission wavelength of 440 nm after an excitation at 330 nm" What is/are the limitation/s of the wavelength/s stated above?

Principles of Instrumental Analysis
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ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
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Chapter21: Surface Characterization By Spectroscopy And Microscopy
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Problem 21.9QAP
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"For scanning, the wavelength range is selected from 400 nm to 200 nm with medium scanning speed since cefuroxime is well-absorbed at an emission wavelength of 440 nm after an excitation at 330 nm"

What is/are the limitation/s of the wavelength/s stated above?

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