For the enzyme urease acting on urea, KM = 2.0 x 10-3 M, and k2 = 2.5 x 104 s-¹ at 298 K and pH 7.5. What is the initial rate of reaction if [urea] = 0.030 mM and [urease] = 5.0 mm, and what is nmax? Assume Michaelis-Menten kinetics apply.

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter34: Miscellaneous Separation Methods
Section: Chapter Questions
Problem 34.19QAP
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For the enzyme urease acting on urea, KM:
2.0 x 10-3 M, and k2 = 2.5 x 104 s-1 at 298 K
and pH 7.5. What is the initial rate of reaction if
[urea] = 0.030 mM and [urease] = 5.0 mm,
and what is nmax? Assume Michaelis-Menten
kinetics apply.
Transcribed Image Text:= For the enzyme urease acting on urea, KM: 2.0 x 10-3 M, and k2 = 2.5 x 104 s-1 at 298 K and pH 7.5. What is the initial rate of reaction if [urea] = 0.030 mM and [urease] = 5.0 mm, and what is nmax? Assume Michaelis-Menten kinetics apply.
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