An enzyme reaction takes place in a 10mL solution that has a total citrate concentration of 120mM and an initial pH of 7.00. During the reaction, 0.2mmoles of acid are produced. (pKa values for citric acid: pKa1=3.15, pKa2=4.77, pKa3=6.40) a) At pH 7.00 (before the enzyme reaction occurs), determine the concentrations of the predominant forms of citrate in solution. b) calculate the pH of the solution after the enzymatic reaction has occurred.

An enzyme reaction takes place in a 10mL solution that has a total citrate concentration of 120mM and an initial pH of 7.00. During the reaction, 0.2mmoles of acid are produced. (pKa values for citric acid: pKa1=3.15, pKa2=4.77, pKa3=6.40) a) At pH 7.00 (before the enzyme reaction occurs), determine the concentrations of the predominant forms of citrate in solution. b) calculate the pH of the solution after the enzymatic reaction has occurred.

Chemistry for Today: General, Organic, and Biochemistry

9th Edition

ISBN:9781305960060

Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Chapter20: Enzymes

Section: Chapter Questions

Problem 20.51E

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