For valine, methionine, GABA and proline, write the zwitterion. Using valine as an example, describe the behavior of the zwitterion in acidic (pH 2) and alkaline (pH 12) media.
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- Pick all that are TRUE regarding analysis of quaternary structures of proteins using polyacrylamide electrophoresis:I. The added β-mercaptoethanol disrupts S--S bonds bridging the polypeptide chains causing the appearance of higher Rf bands compared to the native protein run. II. Heating up any protein before subjecting to SDS-PAGE will always result in the formation of more than one band.III. A good asymmetrical gel layout would be : (Lane 1) MW ladder, (2) native protein, (3) protein + β-ME, (4) protein + HCL, (5) protein + β-ME + HCl.IV. Formation of a single band in the protein + β-ME + HCl run, whose Rf is lower than the native run, could be indicative that the protein is a homodimer.A. I onlyB. I and IIC. II and IIVD. None is true.pls explainA 1.00-mg sample of a pure protein yielded on hydrolysis 0.0165 mg of leucine and 0.0248 mg of isoleucine. What is the minimum possible molar mass of the protein? (MMleucine=MMisoleucine=131g/mol)What is the name of this oligopeptide? Use the three letter and one letter nomenclature of the amino acids. Based on the charges on the peptide, what is the closest pH range would you expect that this was in? 2 to 4? 6 to 8? 10 to 12?
- If the pH of a solution of Arginine is equal to a half of the 3 pka values of all of its functional groups, what will be the charge on the majority of the Arg molecules in that solution? Please explain. Am i supposedd to add up the three pka values and then divide it in half to find the pH? ThanksShow the condensation reactions that result in the formation of the tetrapeptide Asp-Gly-Ser- Gln. After you have finished the synthesis change the structure to show how it would exist in a cell at physiological pH (pH = 7).How does the shape of a titration curve confirm the fact that the pH region of greatest buffering power for an amino acid solution is around its pK's? Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a protein, and describe one condition or reagent that interferes with each type of stabilizing force. Why is silk fibroin so strong, but at the same time so soft and flexible?
- For aspartic and glutamic acids, the isoelectric point occurs at a pH where the net charge on the two carboxyl groups is -1 and balances the charge of +1 on the a-amino group. Calculate pI for these amino acids.For the amino acid alanine, the major species in solution at pH 7 is the zwitterionic form, which has a negatively charged carboxylic acid group and a positively charged amino group. There is a less common neutral form in which neither group is charged. The carboxylic acid group of alanine has a p?apKa of 3.3. The amino group of alanine has a p?apKa of 8.8. Estimate the ratio of the concentration of the neutral amino acid species to the zwitterionic species at pH 7.How would you make 2.0 L of electrotransfer buffer for transferring the proteins from a gel onto nitrocellulose? Its composition is the following in water: 25 mM Tris (this is a solid; MW = 121) 192 mM Glycine (this is a solid; MW = 75.07) 20.0 % Methanol (you have 100% from the stockroom)
- For lysine and arginine, the isoelectric point, pI, occurs at a pH where the net charge on the nitrogen-containing groups is +1 and balances the charge of -1 on the a-carboxyl group. Calculate pI for these amino acids.An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated bypaper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain. Treatment of a new protein with dansyl chloride reveals two (2) dansyl-labelled derivatives of amino acids, alanine and methionine. What can you deduce about the structure of the protein from these results? Name the amino acids that contribute atoms to both purine and pyrimidine rings.How do you draw the titration curve of the tripeptide Met-Lys-Val starting from pH 1 to 12? Do you draw the charged and neutral structures and estimate the relative pKa values? Thanks in advance!