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Compute for CFU/mL (show step-by-step process)
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- With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?why is the recommended incubation procedure for the API 20e system 18 to 24 hours ?
- After incubation of the standard plate count plates, you are instructed to count CFU’s of countable plates. What exactly does countable plates mean?,The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
- "If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)Order: streptomycin 500 mg IM daily for 8 days. If you have vials of this antibiotic that are labeled as 1 g/2.5 mL, how many milliliters would you administer?
- This expiremwnt was carried out . In no less than 30 words what can uconcluded from this labTo prepare a vaginal irrigation, a nurse uses 3 tsp of vinegar and 1.5 L of water. a. How many ml of vinegar should she use? b. If she prepares a solution using only 500 ml of water, how many ml of vinegar should she use?50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?