Frankia isolate EAN1pec respires acetate, but will not grow on acetate as a sole carbon and energy source. The organism will grow on dicarboxylic acids (succinate, malate). The rate of respiration for acetate was equivalent to the rate obtained with succinate. (A) Explain why this isolate does not grow on acetate? (B) Describe an experiment to test your model. (Note: bioinformatics is not an acceptable answer). Hint: Propionate is another compound that it will respire, but not grow on as sole carbon and energy source. Other Frankia isolates including CcI3 will grow on acetate. Respire means oxidize to CO2). (C) Fatty acid synthesis is not simply the reverse of oxidation. What factors distinguish the two pathways from each other? (D) What are anti-sigma factors and how do they function?

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3.
Frankia isolate EAN1pec respires acetate, but will not grow on acetate as a sole carbon and energy
source. The organism will grow on dicarboxylic acids (succinate, malate). The rate of respiration for
acetate was equivalent to the rate obtained with succinate.
(A) Explain why this isolate does not grow on acetate?
(B) Describe an experiment to test your model. (Note: bioinformatics is not an acceptable answer).
Hint: Propionate is another compound that it will respire, but not grow on as sole carbon and energy
source. Other Frankia isolates including CcI3 will grow on acetate. Respire means oxidize to CO₂).
(C) Fatty acid synthesis is not simply the reverse of oxidation. What factors distinguish the two pathways
from each other?
(D) What are anti-sigma factors and how do they function?
Transcribed Image Text:3. Frankia isolate EAN1pec respires acetate, but will not grow on acetate as a sole carbon and energy source. The organism will grow on dicarboxylic acids (succinate, malate). The rate of respiration for acetate was equivalent to the rate obtained with succinate. (A) Explain why this isolate does not grow on acetate? (B) Describe an experiment to test your model. (Note: bioinformatics is not an acceptable answer). Hint: Propionate is another compound that it will respire, but not grow on as sole carbon and energy source. Other Frankia isolates including CcI3 will grow on acetate. Respire means oxidize to CO₂). (C) Fatty acid synthesis is not simply the reverse of oxidation. What factors distinguish the two pathways from each other? (D) What are anti-sigma factors and how do they function?
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