II. The most highly sensitive test in viral hepatitis is y-Glutamyl transpeptidase (Y-GT) increased activity in blood, which level riscs 10-15 times more than the norm (30-50 ME/L). What is the diagnostic valuc of this enzyme? For the answer: a) present the main properties of enzymes which activity determination in patient blood are widely used in enzyme diagnostics; b) explain the y-Glutamyl transpeptidase (y-GT) system and present an appropriate scheme; c) give examples of other enzymes which activity is determined in the liver disorders.
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- 1. What is the major tissue source of Alanine Aminotransferase? 2. What is the clinical significance of elevated serum Alanine aminotransferase?33. Which of the following is an emulsifying agent? a. bile salt b. NarCOs c. both a and b d. neither a nor b 34. What reaction occurs during saponification of triacylglycerol? a. hydrolysis b. condensation c. both a and b d. neither a nor b 36. What was added to the sample prior to heating in acrolein test? a. KHSO4 b. ethanol c. both a and b d. neither a nor b 37. Which of the following is a general test for lipids? a. solubility test b. Huble's test c. both a and b d. neither a nor b 38. Which test can distinguish between sesame oil and corn oil? a. Burchard test b. Baudouin test c. Sudan IV test d. Dichromate test 39. What solvent would be miscible with coconut oil? a. chloroform b. water c. both a and b d. neither a nor b 40. Which of the following is a product of saponification of fatty acids? a. glycerol b. NaOH c. both a and b d. neither a nor bSerum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.
- 1which test is used to differentiate between fermentative and oxidative metabolism?describe the different result that may be observed with this test. 2.Which of the five hydrolysis tests (starch, casein, triglycerides, tryptophan, and urea) would a positive test result be expected for the etiologic agent of acne, Propionibacterium acnes? Explain1. What is the action of acid and alkali on the enzyme in saliva? 2. What is the optimum pH of the enzyme in the saliva?1. What is the role of pyridoxal phosphate in transaminase determination? 2. What is the clinical significance of elevated AST?
- 1. pH cannot affect the enzyme - activity A. True B. False 2. It took 6 mins for the disappearance of blue-black color in test tube 4 with 60 degrees Celsius temperature. A. True B. FalseCimetidine is used in the treatment and management of gastric ulcers and gastroesophageal reflux disease. In the case of an active ulcer, cimetidine can be administered continuous by IV infusion at a dose rate of 37.5 mg/hr. If the concentration of the cimetidine solution is 6 mg/ml, then how many milliliters must be available for a 4-hr infusion?Misoprostol is used to prevent NSAID-induced gastric ulcers. A daily dose consists of two tablets administered four times each day. What is the size of one day’s dose if each tablet contains 100 mcg of misoprostol?2. a. What ingredient(s) necessary to white chocolate does this product have? b. Why is this product labeled as “white chocolate style” instead of being simply labeled as “White chocolate”?
- 41. A 14 year old girl is admitted to the hsopital after taking an overdose of aspirtin. Her plasma salicylate concentration is measured every6 hours for the next 24 hours Hours Plasma Salicylate(mg/dL) 6 12 12 90 18 45 24 10 Which of the following is the most likely explanation for the initial salicylate kinettics observed in this patient (A) Decreased protein binding (B) Delayed Absorption (C) First orderf Elimination (D) impaired Renal Elimination (E) Zero-order elimination1. in cherry crandall method for lipase analysis what is the substrate used? 2. which of the following is the reason why hemolysis shoule be avoided? a. enzymes have lower concentration in cells thus hemolysis increases levels in serum or plasma b. hemolysis does not affect plasma and serum enzyme levels c. enzymes are specific to the target issue d. none of these e. enzymes have higher concentration in cells thus hemlysis increases level in serum or plasma 3. increasing temperature usually increases the rate of a chemical reaction by increasing the movement of molecules. The rate of denaturation increases as the temperature increases and is usually significant at 40C to 50C a. both statement are false b. both statements are true c. first statement is correct, second statement is false d. second statement is true, first statement is false1. Explain the role of L-tartrate in the determination of acid phosphatase . 2. Differentiate thymolphthalein monophosphate method from alpha naphthyl phosphate method of prostatic acid phosphate determination. 3. Why is acid phosphatase determination useful in establishing recent sexual intrusion as in the case of alleged rape?