GTP-y-s is an analog of GTP that cannot be hydrolyzed. How would you expect a Co-IP experiment between G-alpha and G-beta proteins to differ in the presence of GTP-y-a and GDP?
NO answer needs more than 2 sentences, some require just a couple words.
7. GTP-y-s is an analog of GTP that cannot be hydrolyzed. How would you expect a Co-IP experiment between G-alpha and G-beta proteins to differ in the presence of GTP-y-a and GDP?
8. An epithelial cell line expressed a fluorescence-based cAMP biosensor in all cells, but only expressed a GPCR in ~30% of those cells. When you treated the cells with the GPCR ligand, fluorescence from the cAMP sensor immediately turned on in most cells (not just those expressing the GPCR). In one sentence suggest a simple explanation for why cAMP increased in non-GPCR-expressing cells.
9. Taxol is a drug that inhibits microtubule dynamics and is often used for cancer chemotherapy because it preferentially affects dividing cells. What microtubule-based structure is likely disrupted by Taxol so that it specifically affects proliferating cells?
10. Patients with Marfan’s syndrome have a mutation in the fibrillin gene and are at risk of aortic aneurysms. What ECM fibers are compromised in Marfan’s syndrome patients?
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