5. Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine protein phosphatase thatregulates many diverse cellular processes. One study has shown that phosphatidic acid (PA) is aninhibitor of PP1. The investigators measured the activity of PP1 in the presence and absence of theinhibitor PA. The concentration of PA was 300 nM.[Substrate](µM)Vo with PA(µM sec-1)0.00381Vo without PA(µM sec-1)0.0100.02090.0150.03350.006200.0250.04190.009310.0500.08380.0140a. Use Excel to make a Lineweaver-Burke plot showing both the noninhibited and the inhibitedreactions. Your Lineweaver-Burke plot should have labeled axes with units. The graph shoulddisplay both the y-intercept and the x-intercept and include linear trendline equations.b. Determine Vmax and Km for the enzyme in the uninhibited state. Be sure to include your units.c. Determine the type of inhibition for this inhibitor PA. Briefly explain.d. Calculate the K¡ for the inhibitor PA.

Using Lineweaver-Burk plot, we have to find Vmax, Km and Ki for the inhibitor.

5. Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine protein phosphatase that regulates many diverse cellular processes. One study has shown that phosphatidic acid (PA) is an inhibitor of PP1. The investigators measured the activity of PP1 in the presence and absence of the inhibitor PA. The concentration of PA was 300 nM. [Substrate] (µM) Vo with PA (иM sec-1) Vo without PA (иM sec-1) 0.0209 0.010 0.00381 0.015 0.0335 0.00620 0.025 0.0419 0.00931 0.050 0.0838 0.0140

5. Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine protein phosphatase that regulates many diverse cellular processes. One study has shown that phosphatidic acid (PA) is an inhibitor of PP1. The investigators measured the activity of PP1 in the presence and absence of the inhibitor PA. The concentration of PA was 300 nM. [Substrate] (µM) Vo with PA (иM sec-1) Vo without PA (иM sec-1) 0.0209 0.010 0.00381 0.015 0.0335 0.00620 0.025 0.0419 0.00931 0.050 0.0838 0.0140

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter39: Digestion And Nutrition

Section: Chapter Questions

Problem 4CT: Cholera is a disease caused by bacteria that make cholera toxin. In the presence of this toxin,...

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