Answered: Guanosine has a maximum absorbance of…

Guanosine has a maximum absorbance of 275 nm. ϵ275=8400M−1cm−1, and the path length is 1 cm. Using a spectrophotometer, you find that A275=70. What is the concentration of guanosine?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter25: Instruments For Optical Spectrometry

Section: Chapter Questions

Problem 25.11QAP

See similar textbooks

Related questions

Q: How are absorbance and concentration related? Group of answer choices Concentration does not change:…

A: According to the Beer's-lambert law : The absorbance is directly proportional to the concentration…

Q: 1. The dissolved compound in a sample has an absorbance value of 0.250. How would you expect the…

A: As per our answering guidelines, we are supposed to answer Q1. Please ask Q2 separately. Thank you.…

Q: absorbance

Q: A blue product absorbs light at 590 nm (ε590nm = 7,600 M-1 cm-1). Its concentration was too…

A: The actual concentration is 0.459 mM.

Q: Aciortence 2. Use the absorbance-concentration graph given below to find the value of absorptivity…

A: Lambert-Beer Law is utilized to measure the concentration of a sample in a solution using absorbance…

Q: (a) Alizarin (structure shown below) is a red dye used for dyeing textile fabrics. Its extinction…

A: Answer

Q: A scientist has an aqueous solution with an unknown concentration. He uses a 1-cm cuvette and…

Q: Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance…

A: UV-VIS spectrometry gives absorbance values at particular wavelength. This instrument follows Beer-…

Q: A solution of Tryptophan has an absorbance at 280 nm of 0.25 in a 0.5 cm length cuvette. Given the…

Q: Molecule X has a A max at 395 nm in hexanes with a e max = 6000 M²cm'. What is the concentration of…

Q: orgo chem II C-NMR please help with "possible meanings" sections

A: 177ppm can be for carboxylic acid i.e. -COOH group. 34.9ppm can be for alkane 34.9 ppm can…

Q: From graphical analysis of a calibration plot, you generated the line y=2543.23x + 0.0034. Your…

Q: TRANSMITTANCE 6. What functional groups are present in the following IR spectra TE INFRARED SPECTRUM…

A: Ir spectroscopy is used to identfy the functional group of a chemical compound

Q: What

A: The ratio of the absorbance is used to determine the purity of the DNA . If the ratio is around 1.8…

Q: Which of the following relationships between absorbance and %Transmittance are incorrect? correct…

A: Spectrophotometer is a device consists of monochromator that produces a light beam containing a…

Q: You are using mass spectrometry to determine the molecular weight of a peptide, which has multiple…

A: Mass spectrometry is used to determine the m/z ratio of the ions present in a sample. These ions are…

Q: constant obtained in this experiment, calculate the absorbance of the solution of 0.0600 M CoCl2. My…

A: Absorbance=ECl E is beers law constant l =1

Q: 2. What is the concentration (mM) of a solution of tryptophan if the absorbance at 280 nm is 0.850?…

Q: Convert the 98% T to absorbance: a. 0.008 b. 0.08 c. 0.021 d. 0.21

A: A (absorbance) = - logT T = transmittance... Rule, A = 2 - log (T%) Given T% = 98

Q: Which of the following is FALSE about the absorbance of a compound? O A. Organic solvents have…

A: Ans. (A). In organic chemistry, solvents can have an effect on solubility, stability and reaction…

Q: Rank these colors from the best to the least absorber of radiation. (Best absorber - I; Least…

A: The color which absorbs least of radiation is white. The color which absorbs most of radiation is…

Q: Given the following standard curve of Absorbance vs Concentration (mM), what in the concentration of…

A: Given : y = 0.0032x + 0.1043

Q: 3. The chemical sunset yellow, also knowm as yellow #6, looks yellow. What color(s) must be absorbed…

Q: Absorbance Transsmission 0.730 17.8 0.641 22.8 0.400 31.8 0.340 45.2 0.211 62.0 0.076 85.4 0.020…

A: Given:

Q: What is the absorbance of a theoretical compound in a 5.26 x 10-5 M solution, a path length of 1.5…

A: The relationship between the absorbance and concentration is given by A = ECl where A = absorbance…

Q: Caffeine (C8H10O2N4•H2O) had an absorbance of 0.510 to 272 nm in 1.0 cm optical path in…

A: Given: Concentration of caffeine, (C) = 1.0 mg/100 ml. The molar mass of caffeine, (M)=212.09 g/mol.…

Q: Look at the absorbance spectrum shown below. The value of lambda max you should report from this…

A: Lambda max is the wavelength of maximum absorbance.

Q: Draw a plot having X-axis as concentration (mol/L) and Y-axis as absorbance at λ max (525 nm).…

A: 1. Concentration vs absorbance plot

Q: solution with %T = 75.9% has an absorbance equal to: A = log (1 T) T= (% T 100)

A: Relationship between absorbance and transmittance, A = - log T And Relationship between absorbance…

Q: The drug tolbutamine (mw=270) has a molar absorptivity of 703 at 262 nm. One tablet is dissolved in…

A: According to the Beer-Lambert’s Law: where A is the absorbance of the solution, ἐ is the molar…

Q: If the concentration of a food coloring solution is increased by a factor of 3, how will the…

A: According to Beer Lambert law, the absorbance of any solution is directly proportional to the…

Q: A specific steroid has a Amax of 258 nm and a molar absorptivity e of 11, 500 L mol- cm-. What is…

Q: Calculate the absorbance of the vitamin solution at 324 nm (sample pathlength = 1.00 cm)

A: ** This question can be solved with the help of Beer - Lambert's law **Beer - lambert's law states…

Q: Based on the plot, what [Fe(SCN)2+] would the student report for a solution that has an absorbance…

A: The given plot shows the plot of Absorbance versus concentration, where the absorbance of the…

Q: To measure the electronic spectrum of a product that absorbs in the near UV region (260 nm) we will…

A: Two kinds of lamps, a Deuterium for measurement in the ultraviolet range and a tungsten lamp for…

Q: A low-resolution mass spectrum of the neurotransmitter dopamine gave a molecular ion at m/z = 153.…

A: A low resolution mass spectra gives the molecular mass of a compound to the nearest whole number.A…

Q: How do I find the theoretical molarity?

A: The values are given as follows:

Q: A scientist has an aqueous solution with an unknown concentration. He uses a 1-cm cuvette and…

A: Absorbance (A) of a solution at a specific wavelength is proportional to concentration (C) of…

Q: Why does Cytochrome C have a peak absorbance of 409nm? When proteins usually have a peak absorbance…

A: The cytochrome C is a small heme-protein which is found loosely with the inner membrane of the…

Q: The mass spectrum of compound B, a colorless liquid, shows these peaks in its mass spectrum.…

A: The ratio of the molecule M to M+2 peaks is 216/7.55=3.13 to 1. This is approximately the same ratio…

Q: 4. Using this formula Cu = Au x Cstd. Astd Compute for the concentration of the uric acid of the…

A: Given, Absorbance of unknown (Au) = 0.241 Absorbance of standard (Astd) = 0.109 Concentration of…

Q: You measure a sample in your spectrophotometer that has an absorbance of 1.00. What is the…

A: The relation between absorbance and concentration which results from the graph is as follows:…

Q: What type of compound corresponds to the IR spectrum shown here? MICRONS 25 2.62.72.82.9 3 100 3.5…

A: IR spectrum is used to study and identify chemical substances or functional groups present in them…

Q: What is a characteristic feature of halogens on the mass spectrometry spectra? A. They have…

A: Halogens have special characteristic feature in mass spectra. Chlorine atom has isotopes 34.97 and…

Q: Chemistry Name the compound using mass spec, NMR, and IR.

A: From the mass spectra, it is given that the mass of the unknown compound is 245.32 According to the…

Q: Using a 96 well plate, you measure the absorbance of a 200 µL sample at 655 nm. You obtain the…

Q: Calculate the concentration (in ppm) of each compound that would provide an absorbance value of 0.50…

A: Lambert beer law When a monochromatic light is passed through a solution so the absorbance A of the…

Q: The development of hyphenated techniques has resulted in reduction of sample requirements, shorter…

A: Hirschfeld presented the expression hyphenation to allude to the online blend of the partition…

Q: The aspirin an analgesic drug forms a complex with metal ion which has a molar absorptivity of 8000…

Q: A specific steroid has a max of 254 nm and a molar absorptivity & of 11, 500 L mol-¹ cm-¹. What is…

Question

100%

Guanosine has a maximum absorbance of 275 nm. ϵ275=8400M−1cm−1, and the path length is 1 cm. Using a spectrophotometer, you find that A275=70. What is the concentration of guanosine?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Similar questions

After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40 and processing her data, Sally found that her drinking strength Kool-Aid sample had a concentration of 4.692 x 10–5 M. If 0.132 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her Kool-aid powder? Report your answer as a percentage with two places after the decimal. Do not include the percentage sign in your answer. Pay attention to units. You are trying to find the mass percentage of red dye, so you need to know each variable in the equation in Appendix B. Note that the percent by mass equation should be multiplied by 100. There may be some useful conversion factors in the experimental procedure.
From the following data, calculate the concentration of the analyte in the sample read at 700 nm: Absorbance of unknown sample = 0.807 Absorbance of a 130 mg/dl standard = 0.234 Do not answer in image format. Maintain accuracy and quality in your answer. Answer completely.
The equation of your standard curve of absorbance versus concentration (mM) is y = 1.245x + 0.0012. You put a solution of unknown concentration into the Spec20 and get an absorbance value of 0.227, which lies within the standards used to make the standard curve. What is the concentration of the unknown solution in mM?
Chemistry The most abundant type of mammalian antibody circulating in the blood stream is IgG. A solution prepared from 0.798 g of a particular IgG in 250 mL of water shows an absorbance of 0.578 at 275 nm in a 1.00 cm cell. If the molar absorptivity of the IgG is 6.49 × 104 M–1 cm–1 at 275 nm, what is the molar mass of the IgG? Present your numerical answer in kg·mol-1 to 3 significant figures .
The following data were obtained for 1.00 cm samples of a particular chemical. What is the concentration of a 1.00 cm sample with an absorbance of 0.60 according to the graph below? Round to 2 decimal places do not include units!
A solution of a chemical compound absorbs at 350 nm. What is the concentration of this chemical compound? Transmittance of the solution: 0.63 Transmittance of the blank: 0.91 Pathlength: 1.0 cm e at 350 nm 18,400 M-1cm-1
What is the importance of the following analytical methods in chemical analysis? i. UV-vis spectrophotometry ii. HPLC iii. GC
Guanosine has a maximum absorbance of 275 nm. ϵ275= 8400M−1cm−1 and the path length is 1 cm. Using a spectrophotometer, you find the that A275=0.70. What is the concentration of guanosine?
After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40, a student found that her best fit linear line for FD&C Red 40 was y = 2,962x + 0.005. Her Kool-Aid sample had an absorbance of 0.685. If 0.543 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her 8-fl oz cup?
The trendline for a calibration curve is y=4.299x+-0.0001. The absorbance of a solution of the unknown concentration is 0.0812 What is the concentration of the unknown solution in uM
A solution prepared by dissolving 25.8 mg of benzene (78.11 g/mol) in hexane (86.16 g/mol) and diluting to 250 mL had an absorption peak at 256 nm and an absorbance of 0.266 in a 1.000-cm cell. If a sample of hexane with benzene had an absorbance of 0.153 at 256 nm in a cuvette with 1.000-cm path length, find the concentration of benzene in molarity. A. 1.51×10-4 M B. 7.59×10-4 M C. 5.41×10-4 M D. 6.69×10-4 M
A plot of Absorbance vs. molarity of Fe(SCN)2+ from part one of the lab gives a straight line with the following equation. y = 51x R2 = 0.998 If a student measures an absorbance value of 0.375 in the second part of the lab, what is the molarity of Fe(SCN)2+ in this solution?