Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance can be calculated by: A A cm 1% 1 ст c(1% For the identification of a substance, 0.0482 g of substance has been weighed, which has been dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925. %3D a) Calculate the concentration of the diluted solution in% (w/v). b) Calculate the specific absorbance of the substance. c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why /why not?

Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance can be calculated by: A A cm 1% 1 ст c(1% For the identification of a substance, 0.0482 g of substance has been weighed, which has been dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925. %3D a) Calculate the concentration of the diluted solution in% (w/v). b) Calculate the specific absorbance of the substance. c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why /why not?

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter20: Molecular Spectroscopy And Photochemistry

Section: Chapter Questions

Problem 1P

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