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How can you make a final dilution of 10-10 using 5 dilution tubes? Outline your steps.
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- What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?
- How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?After performing a plate and liquid lysate, it was found that the plate lysate achieved the common titre range of 1010-1011 pfu/ml whereas the liquid lysate achieved a range of 108 PFU/mL; what is the reason for the lower titre in different methodologies? How can we troubleshoot this issue in the future?
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a sterile petri dish and then added molten agar to make a pour plate?how did you get 104 as the dilution factor? I got 10-2 as the dilution factor for the CNFL plate
- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?i. Illustrate the dilution series used and label the final dilution of each dilution.What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).
