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how do you do the calculations for rate of diffusion for this table
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- It refers to the general term of adsorbent media used to visualize chromogenic compounds.A. ChromatogramB. Solvent Front C. Mobile PhaseD. Stationary PhaseSuppose that the sample size was 3.0 mg, the column dimensions were 1.00 m length 3 4.0 mm diameter, and the flow rate was 7.0 mL/min. What size column and what flow rate should be used to obtain the same quality of separation of 27.0 mg of sample?Column length: 25.0 cm Column diameter: 0.46 cm Stationary phase: C-18 Mobile phase: 80% methanol in water Flow rate: 1.0 mL/min Dead time: 5 min Peak width at base, WA = 20 Peak width at base, WB = 24
- Prepare a procedural flow chart that provides a simplified overview of the entire extraction process.How do you calculate the retention factor (Rf) for each spot ? Distance traveled by spot O 5.5cm Distance traveled by spot Y 4.0cm Distance traveled by spot G 3.0cm Distance traveled by solvent front 6.0cmA solute with a retention time of 400.0 s has a width at half-height of 8.0 s on a column 12.2 m long. Find the number of plates and the plate height.
- kindly prepare an assignment on Application of HPLC in Drug Analysis: one page show name of the drug, dosage form, extraction step, type of column, separation parameters e.g., flow rate, mobile phase, detection wavelength and run time. Submit before 05.11.On a paper chromatogram, a spot produced by blue ink traveled 2.7 cm, and the solvent "front" traveled 5.7 cm during the experiment. Calculate rate of retention of flow, Rf, for the blue ink.Define the following terms used in HPLC: (d) sampling loops (e) bonded-phase packing (f) gel filtration
- Which statement is valid when the multiple extraction and single extraction is compared. The effciency of multiple extraction and single extraction is incomparable. Single extraction and multiple extraction have the same effciency Multiple extraction is more efficent than single extraction Single extraction is more efficient than multiple extractionin the separation of these 8 organic herbicides using HPLC in two different modes: Isocratic elution and gradient elutions. Stationary phase is C18 and the eluent is water-acetonitril (ACN) mixture as presneted in the figure. Rate is the same (1ml/min, Temperature is the same 35 C). i) Compare both the isocratic elution and gradient elution in this experiment. ii) explain why component 8 comes at 30 min in gradient elution without peak tailing while it appears at ~ 65 min in isocratic elution as shoeter and wider peak with tailing.A supporting electrolyte is usually added to the analyte solution to minimize solution resistance.minimize solution conductivity.make the analyte less soluble in solution.increase the scan rate.