Answered: how does colony appearance and location…

how does colony appearance and location with respect to the agar differ on the spread and pour plate? 2. list two additional factors that contribute to error in the spread plate method:

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 5RQ

See similar textbooks

Related questions

Q: A turkey farmer is worried about a new disease that is found in his birds. If affects females more…

A: It is given that A disease is found in turkeys Disease predominantly affects female turkeys than…

Q: 4. What is coevolution? Provide an example of predatory-prey coevolution.

A: One such instance of coevolutionary dynamics is the evolution of predators and their prey. As a…

Q: An afterimage is a visual experience that can be explained by combining. principle of trichromatic…

A: An afterimage is a visual experience where a person continues to see an image after the original…

Q: Draw or describe what a rod-shaped bacterium positive for a capsule would look like when stained…

A: capsule stain is a useful method in microbiology for identifying the presence of capsules, the…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: In wild-type E. coli, the cAMP receptor protein (CRP) binds to cAMP and activates transcription of…

Q: Match the following situation with the appropriate nutrient with which it might be associated. A.…

A: Introduction to Vitamins and Vitamin DeficienciesVitamins are essential organic compounds that our…

Q: Which is a difference between B-DNA and A-DNA? A. For polynucleotide strands containing the same…

A: DNA:- Only acts as genetic material- Polynucleotide (monomeric unit is nucleotide)- Nucleotide is…

Q: The oldest known of the sites listed below that has modern human remains is____. a. Kabul b. Klasies…

A: The question relates to identifying the oldest known site with modern human remains among the given…

Q: Heartburn is a form of indigestion felt as a burning sensation in the chest when stomach acid comes…

A: Given informationHeartburn is a form of indigestion felt as a burning sensation in the chest when…

Q: Name the three major assumptions made by the "Cell theory". (i) The lipid membrane is composed of…

A: The "Cell theory" is a fundamental principle in biology that describes the basic characteristics of…

Q: The pedigree below is of a rare single gene disease. Given the pedigree shown below, what is the…

A: Pedigree analysis is the study of inheritance patterns within a family by examining the family tree,…

Q: Homologies Both mammals and birds are descendants of ancestral reptiles. Meaning, they both…

A: Analogous structures are biological structures with similar functions that arose independently in…

Q: 1st Bag: shredded wheat original cereal, Warm water, and yeast (no sugar) 2nd Bag: Cinamon Toast…

A: Fermentation is a fundamental biological process that has been harnessed by humans for centuries to…

Q: (4521) Ahdl (3628) Peit (1001) Scal (4801) Pul 4000 (3249) Bt2171 (3197) Bam (5325) Sep 5000 Ampl…

A: The correct answer is (b), anti-myc.

Q: 2. Explain why dispersal behaviour shown amongst majority of species improves reproductive success.

A: Dispersal is a process that mainly involves the movement of specific individual or multiple…

Q: You're purifying some plasmid DNA from a culture of bacteria and you want to know how pure it is.…

A: The purity of the resulting DNA must be assessed when plasmid DNA is purified from a bacterial…

Q: can you tell me what group of viruses (groups 1-7) the image represents and explain how you know?

A: Microorganisms known as viruses can infect various hosts, including people, plants, and animals.…

Q: Please help me with the multiple choice question below - it is based on genomics/developmental…

A: The correct answer is E. Transcription factors.

Q: [Select] Table 2 [Select] Xgal glucose > Xgal + lactose [Select] [Select] Minimal lactose ΝΑ >…

A: In the laboratory setting, it is common to study and analyze various mutants of microorganisms to…

Q: In a certain ecosystem, field mice are preyed on by snakes and hawks. The entry of wild dogs into…

A: Ecological systems are composed of complex interactions between organisms and their environment.…

Q: 9. Given the following table of traits, which of the following is/are the most parsimonious tree(s)?…

A: There are different approaches which are used for classification of organisms. The two broad…

Q: 아 70 60 50 4000 3297 3296 3072 2997 2996 3000 Wavenumber [cm-11 2000 1635 1630 1536 1447 1448 1534…

A: FTIR is used in understanding and identifying organic, inorganic and polymeric compounds in a given…

Q: write an abstract regarding the two diffrent studies provided

A: In this research study, two different experiments were conducted to investigate the phenomenon of…

Q: 13. BbCC x bbcc (B= Brown eyes, C = Curly hair) a. BbCC: F= b. bbcc: F= Gametes →>>> ↓ c.…

A: The genotype of an individual is their own DNA pattern. In particular, the two alleles a person…

Q: The Table below shows the names of proteins whose functions are regulated through the binding of…

A: The table lists proteins that have specific ligands that regulate their functions. Ligands are…

Q: Many plants are adapted to survive harsh conditions . Describe two modifications found in the stems…

A: Cacti are a type of succulent plant that belong to the family Cactaceae. They are native to arid and…

Q: 1. Compare the survival and reproduction strategies of altricial species and precocial species.…

A: Altricial species refer to a category of animals whose offspring are born or hatched in a relatively…

Q: can you tell me what group of viruses (groups 1-7) the image represents and explain how you know?

A: The categorization of viruses depends on several factors. Some of the factors involve genetic…

Q: 1. Which of the following is most true about interspecific competition? a. It involves interaction…

A: Interspecific competition refers to the ecological interaction between different species in which…

Q: A. What is research

A: Note: Please upload your other questions separately as according to the answering policy the tutors…

Q: 1st Bag: shredded wheat original cereal, Warm water, and yeast (no sugar) 2nd Bag: Cinamon Toast…

A: In this experiment, three different bags were prepared, each containing a different type of cereal,…

Q: 1. You are a lead engineer in a cGMP facility that develops and delivers tissue engineering…

A: The creation of injectable treatments for replacing damaged heart tissue offers considerable promise…

Q: Which of these characteristics is shared by ferns and gymnosperms (select all that apply)? vascular…

A: The following characteristics are shared by ferns and gymnosperms:Vascular TissuePollenChloroplasts

Q: A serotonin can bind to ___. A. All nuerotransporters B. Serotonin and glutamate receptors since…

A: Serotonin is an essential neurotransmitter and hormone that affects many physiological and mental…

Q: Propose a model that outlines, step-by-step, events that could generate the gradients of…

A: Spatial regulation of gene expression plays a crucial role in embryonic development, ensuring the…

Q: Identify the feature of the heart labeled "7" in the picture. A Identify the chamber of the heart…

A: The heart is a muscular organ located in the chest that serves as the central pump of the…

Q: 4. Describe and give an example of each of the following: a. Commensalism b. Mutualism c. Parasitism

A: Commensalism is a symbiotic relationship in which one organism benefits, while the other is neither…

Q: Which type of adrenergic receptors would promote glycolysis in response to epinephrine/ adrenaline…

A: Beta-adrenergic receptors specifically the Beta-2 adrenergic receptors promote glycolysis in…

Q: Construct a table that evaluates the effects of oestroge, testosterone and progesterone on the…

A: Reproductive system is one of 11 body system which is involved in reproduction , formation of sex…

Q: What do you think might occur if we do not shift our nutritional consumption to best support the…

A: Our body is mainly composed of six basic elements which are oxygen,…

Q: Original: Met / Pro / Thr/ Asp / Trp / Pro Mutated: c. Silent Mutation Original: 5' AUG / CCU / ACG…

A: Any abrupt changes in the sequence of DNA nucleotideAny abrupt changes in the sequence of DNA…

Q: 1.Identify the three taxa that form a monophyletic group. 2. Identify the three taxa that form a…

A: Phylogenetic groups, also known as clades, are classifications of organisms based on their…

Q: Silent mutations do not lead to deleterious effects to an organism. Provide examples of when a…

A: It is sudden heritable change in genetic material of organisms. The termn mutation was coined by…

Q: What is the study of Ecology in Early Childhood

A: Ecology in early childhood in more detail:1. Introduce basic ecological concepts: Start by…

Q: If the concentration of a solute is the same both inside and outside the cell, what might you expect…

A: Note: Please note that keeping with the site regulations and in order to provide detailed and…

Q: You are looking at a slide from a patients sole. Which of the following layers of the epidermis…

A: Sole is the undersurface of a foot. This is present in the bottom of the foot. This can called as…

Q: What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units.…

A: Electrophoresis is the technique to separate biomolecules depending on their…

Q: 3. BbXCX x bbXY ***B a. BbXCX: F= b. bbxSY: F= Gametes c. Phenotypes: = Brown eyes, XS = Colorblind…

A: The parents are BbXCXc and bbXcY.Colour blindness is a genetic disorder and is sex linked.The genes…

Q: Which of the formed elements from blood participate in the clotting process? Platelets Leukocytes…

A: Blood is a type of connective tissue. Blood plays various functions in our body. RBC of our blood…

Q: 8. Clonal Selection Hypothesis is the most accepted theory for how immune cells respond to specific…

A: Immune cells, also known as immune system cells or leukocytes, are specialized cells that play a…

Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

1. how does colony appearance and location with respect to the agar differ on the spread and pour plate?

2. list two additional factors that contribute to error in the spread plate method:

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: Introduction

Step 2: Explanation

Step 3: Explanation

Solution

Step by step

Solved in 4 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.
1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…
1. What is the statistically significant number range of colonies that may be counted on a Petri dish (for the pour plate method)? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______ 3. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture?
The main of objective of using 13-Line streaking method is to get: A-Contaminated cultures B-None of the above C-Overlapped colonies D-Separated colonies
(1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific
1. if 250 colonies are present on a pour plate made from 1:10^5 dilution, how many colonies should grow on a plate made from 1:10^6 dilution? Explain? 2. list two additional factors that contribute to error in the pour plate method:
1.What would happen if an error occurs in a serial dilution? 2.Why does one cell not necessarily mean equal to one colony? 3. What would happen if incubation time of inoculated agar plates is too short or too long? 4.Why is viable plate count so convenient to apply when counting bacteria?
1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
1. Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How many colonies would you expect if you plated out 0.1 mL from Tube C. c) How many colonies would you expect if you plated out 1.0 mL from Tube C.
1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above
Explain shortly the concept of differential centrifugation and how you would limit pellet contamination which is a disadvantage of this technique.
What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreader