How does proofreading improve replication fidelity?

Q: What observation in the Meslson and Stahl experiment to decipher the DNA replication model…

A: There are three models for DNA replication :-  A ) Conservative  B ) Semi conservative  C )…

Q: What are the differences between a DNA library and acDNA library?

A: For the gene cloning method, the DNA is used, which is cut and ligated in the vector. This…

Q: Can you explain what semiconservative means in relationship to the way DNA is copied?

A: In the process of DNA replication, two daughter DNAs are formed with one old and one new strand…

Q: Explain Proofreading by DNA polymerase.

A: A DNA polymerase is an enzyme that catalyze the synthesis of DNA molecules from nucleoside…

Q: what are the purpose of sequence alignment? define local alignment and global alignment?

A: Alignments are an effective method for comparing similar DNA or protein sequences. DNA molecules are…

Q: Why Sanger Sequencing Dependson DNA Polymerase?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: What modifications would be recommended for easy, optimized, yield and quality DNA when plant cells…

A: Plant materials are among the most difficult materials when it comes to quality DNA extraction due…

Q: The synthetic unit of the polymerase chain reaction is the replica. True or false?

A: Introduction  PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…

Q: How can you modify DNA model to reflect changes among DNA molecules?

A: DNA carries all the genetic and phenotypic information about the body and it is found in the genes,…

Q: What is meant by “proofreading” with respect to DNA polymerase?

A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…

Q: What is the difference between positive and negative supercoiling?

A: The DNA (Deoxyribonucleic acid) is the genetic material that is inherited by offspring over…

Q: How does gel electrophoresis separate different-sized DNA fragments?

A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…

Q: How does proofreading take place in the process of DNA replication?

A: DNA replication is the process of producing two identical replices of DNA from one original DNA…

Q: How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?

A: Deoxyribonucleic acid (DNA) is a particle made out of two polynucleotide chains that loop around one…

Q: Why is it a large undertaking to construct a DNA library?

A: A deoxyribonucleic acid library could be an assortment of cells that carry cloned items of the whole…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: Explain why accuracy is more important for DNA replication than for transcription

A: DNA replication is that the method by that a double-stranded DNA molecule is traced to provide 2…

Q: Explain the proofreading function of DNA polymerase.

A: Proofreading means correcting the incorrect base pairs by removing and adding correct base pairs.…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: Explain the mechanisms on the proofreading of DNA.

A: Biological macromolecules are those large molecules that are necessary for the survival and growth…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: Does proofreading always take place by the same process in replication?

A: REPAIR PATHWAYS:- Both in Prokaryotes and Eukaryotes, there is a repair enzyme system to deal with…

Q: What is meant by semi conservative replication? How did Meselson and Stahl prove it  experimentally?

A: DNA is the information hub of the cell that contains instructions for the synthesis of proteins. The…

Q: Why does one need a vector to clone a DNA

A: Cloning is the method of generating the number of copies of desired DNA I  the host.

Q: What is the replisome and what does it contain?Why can replication occur faster on circular DNA than…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: The major products of proofreading by RNA polymerase are dinucleotides rather than mononucleotides.…

A: Introduction: DNA polymerase holds the ability to proofread the strand synthesized during…

Q: What are at least three molecules involved in DNA transcript proofreading that we reviewed? What are…

A: In humans the DNA is highly coiled to form chromosome. The DNA has many genes which can encodes a…

Q: what are some Limitations/sources of error with suitable rationalization that may occur during the…

A: PCR is in vitro method for amplifying defined target DNA sequences present within the source of DNA.…

Q: How can specific genes be retrieved from a DNA library?

A: Deoxyribonucleic acid or DNA is a molecule that contains the genetic code of the organisms. DNA…

Q: What is DNA microarray used for?

A: It is a laboratory tool used to detect the expression of thousands of genes at the same time. The…

Q: What is a DNA library? Do you think this name is appropriate?

A: In terms of molecular biology, a library can be defined as the collection of DNA fragments that are…

Q: What is a good transformation efficiency?

A: Transformation is a common phenomenon in microbes like bacteria and results in genetic alteration of…

Q: What is an advantage of RNA Seq over microarrays?

A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…

Q: How does a DNA microarray work amd How do you make a DNA microarray?

A: DNA microarray is a collection of DNA sequences attached to a solid surface such as a glass slide…

Q: What are replication licensing factors? How did they get their name?

A: Replication is a process by which the DNA is duplicated to form two identical copies of DNA using…

Q: Hi, I would like to know which program is used for the graphical presentation of the results of a…

A: A genome wide linkage association study (GWAS) is an approach used in genetic research to associate…

Q: What factors could explain a transformation efficiency?

A: Transformation efficiency is the efficiency with which a bacterial cell can take up the…

Q: What is the primary disadvantage of Sanger sequencing?

A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…

Q: Explain the advantages of DNA microarrays ?

A: A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Each DNA spot…

Q: What is the meaning of proofreading activity ? O A. The polymerase checks for the correct…

A: DNA means deoxyribo nucleic acids. DNA act as genetic material in most organisms present on earth.…

Q: Describe the proofreading activity of DNA polymerase.

A: Although DNA polymerase is a very effective enzyme, it can potentially introduce an erroneous…

Q: In the Meselson–Stahl experiment, which of the three modes of replication could be ruled out after…

A: The central dogma of molecular biology represents the flow of genetic information from DNA to RNA to…

Q: How does proofreading differ from later DNA repair mechanisms in terms of timing and the proteins…

A: Proofreading done by DNA polymerase.DNA polymerase detect error at the time of replication and also…

Q: Describe at least three proof-reading mechanisms to achieve High Fidelity of DNA replication.

A: DNA proof-reading is a process of error correction in DNA. It is also called as the DNA repair…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.