Q: a. What are the purposes of sequence alignment?
A: Sequence alignment is a method by which one can arrange sequences of DNA, RNA and protein so as to…
Q: What is the ENCODE project?
A: ENCODE is Encyclopedia of DNA Elements. It is a project funded by National Human Genome Research…
Q: How are dideoxynucleotide triphosphates utilized in sequencing?
A: DNA is the genetic substance of all creatures. It is a double-helical structure made up of two…
Q: What do you need to pay attention to when designing SNP specific primers for the detection of gene…
A: When we designing SNP specific primers for the detection of gene sequences pay attention on…
Q: what is the main purpose of performing the bioinformatics analysis of 16s rRNA genes lab?
A: Bioinformatics: The application of computation and analysis tools to the capture and interpretation…
Q: How do you verify amino acid sequences with proteins and species with BLAST?
A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…
Q: What do the spacers within the CRISPR region correspond to?
A: CRISPR that is clustered regularly interspaced short palindromic repeats. It is found in the…
Q: Briefly outline the components of the CRISPR/Cas system
A: Nonspecific endonuclease (Cas9 or Cpf1 closely associated) is used by CRISPR / Cas9 genome editing…
Q: What are the possible bioethical issues that gene editing tools may encounter?
A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…
Q: What are gene microarrays? How are microarrays used?
A: The process of forming a single double-stranded nucleic acid molecule by combining two…
Q: what is a palindromic sequence?
A: If a nucleic acid sequence in a double stranded DNA or RNA molecule is read in a particular…
Q: What is domain? And Why is it complicated to classify and study domains purely using sequence…
A: All the prokaryotic organisms belong to Archea or Bacteria while eukaryotic organisms belong to…
Q: Why a multiple sequence alignment is needed for researchers? What inferences can be derived from…
A: When three or more biological sequences are aligned and studied together, it is referred to as…
Q: In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?
A: Ribonucleic acid sequencing (RNA-seq) was developed by Michael Snyder and colleagues in 2008. It is…
Q: What is oligonucleotide microarray?
A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…
Q: How BLAST Searches Automate the Findingof Homologous Sequences?
A: Basic local alignment search tool (BLAST) is an algorithm and program for comparing primary…
Q: Why are the proteins cleaved into small fragments for protein sequencing?
A: Proteins are the end product of the molecular translation that codes the information present in the…
Q: What is the difference between RefSeq and GenBank?
A:
Q: What does 'biological sequence database' refer to?
A: Bioinformatics aids in the storage and process of large biological information by the aid of…
Q: Explain the sequencing-by synthesis (SBS) approach ?
A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…
Q: Use the sequence alignment tool Smith and Waterman for the amino acid number 16-30 of the protein…
A: The Smith-Waterman algorithm uses local sequence alignment to find similar regions between two…
Q: What is the principle behind to Sanger sequencing?
A: Sanger sequencing is a method of DNA sequencing, which is based on selective incorporation of…
Q: Draw the structure of a typical insertion sequence and identify its parts.
A: There are two types of transposons such as replicative and non-replicative transposons. They are…
Q: Outline different strategies for identifying gene sequences.
A: Genome is the study of all genes present in the organism. Gene is a unit of heredity that is…
Q: How to find unknown gene and design primers present using whole genome sequence?
A: Step 1 Whole genome sequencing is the process that regulates the complete deoxyribonucleic acid…
Q: What are gene coordinates?
A: Genes are the basic structural and functional units of heredity. They carry coded genetic…
Q: What is a read (Sanger sequencing)?
A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…
Q: How are different variable region sequences generated?
A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…
Q: Explain how substitution matrices can be used to improve the possibility of identifying related…
A: Protein is a macronutrient that is vital for building muscle mass. It is normally found in creature…
Q: Why is GenBank important and What is GenBank format?
A: GenBank is the Genetic sequence database at NCBI (National center for biotechnology information). It…
Q: What is Central Dogma of Molecular Biology and how this concept is used in the development of…
A: Genetically modified organisms are those that have been modified by humans in order to show those…
Q: describe what is s autonomouslyreplicating sequences?
A: Yeast is eukaryotic, single-celled microorganism classified as members of the fungus kingdom. The…
Q: What is an advantage of RNA Seq over microarrays?
A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…
Q: What is CRISPR?
A: Introduction Bacteria and viruses are tough competitor to each other, in order to overcome each…
Q: What are frame-shift insertion?
A: Mutations are changes that occurs in the deoxyribonucleic acid (DNA) sequence, either due to…
Q: Explain how Sanger sequencing works
A: Genome sequencing: genome means total gene content present in the organism and sequence means to…
Q: What is a short tandem repeat (STR) and which method do we use to detect it and which part of the…
A: Short-term repetition of tandem (STRs) is a short-lived sequence of DNA (2–6 bp) that accounts for…
Q: What are the main differences between bottom up/shotgun and top down proteomics strategies?
A: Proteomics studies plays an important role in biomarkers and drug targets. Mass spectrometry (MS) is…
Q: Why is sequence alignment critical to phylogenetic analysis?
A: Sequence alignment: It is an arrangement of the sequence of protein, RNA, and DNA. It is essential…
Q: What is a genome and what is it composed of? What is thecentral dogma of molecular biology?
A: Genes are the hereditary units that are transmitted from one generation to another generation. The…
Q: Explain the use of Annotation to Identify Gene Sequences ?
A: The attachment of biological information to the gene sequence is known as genome annotations. Three…
Q: Describe how Ion torren method of sequencing works in detail.
A: Thermo Fisher Scientific's Ion Torrent technology brings a whole new method to Next Generation…
Q: What key molecules are essential for Sanger sequencing?
A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…
Q: List down and briefly describe three other sequence alignment tools other than BLAST used in…
A: BLAST can rapidly align and compare a query DNA sequence with a database of sequences, which makes…
Q: If you have access to the necessary computer software, make asequence file and analyze it in the…
A: The sequencing of genetic information is important for proper study and analysis, and with the…
what are the purpose of sequence alignment?
define local alignment and global alignment?
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Solved in 2 steps
- How to create DNA-sequence alignment ?What is meant by the term "alignment" in genomic research and in what ways does alignment aid sequence assembly? How can relying on alignment lead to potential errors?What is a repetitive element in genomics? What are the types of repetitive elements? What is their effect on the ease of determining and analyzing a genome sequence?
- Outline different strategies for identifying gene sequences.What is the primary disadvantage of Sanger sequencing?If you have access to the necessary computer software, make asequence file and analyze it in the following ways: What is thetranslated sequence in all three reading frames? What is the longest open reading frame? Is the sequence homologous to any known sequences? If so, does this provide any clues about the function of the sequence?