What is the replisome and what does it contain?Why can replication occur faster on circular DNA than onlinear DNA? What is proofreading and why is itimportant?
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What is the replisome and what does it contain?
Why can replication occur faster on circular DNA than on
linear DNA? What is proofreading and why is it
important?
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- What is an Okazaki fragment? In which strand of replicating DNA are Okazaki fragments found? Based on the properties of DNA polymerase, why is it necessary to make these fragments?What observation in the Meslson and Stahl experiment to decipher the DNA replication model distinguishes the semi-conservative from the dispersive mode of replication? Explain.When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?
- How does proofreading differ from later DNA repair mechanisms in terms of timing and the proteins involved?The chromosome of E. coli contains 4.6 million bp. How long will it take to replicate its DNA? Assuming that DNA polymerase III is the primary enzyme involved and that it can actively proofread during DNA synthesis, how many base pair mistakes will be made in one round of DNA replication in a bacterial population containing 1000 bacteria?What is the role of Mg2+ in genomic DNA extraction? Why is it important to dissolve DNA in TE buffer after we extract it?
- Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the leading strands? In PCR, we also start with an antiparallel strand of DNA. Does this also mean that replication is semi-discontinuous in in vitro replication?What key structural features of the DNA molecule underlie its ability to be faithfully replicated?how can denaturing environment be created during electrophoresis? which bonds are broken during DNA denaturation?
- What are cis acting DNA sequences? List themHow often, on average, will the endonuclease AluI, which cleaves the sequence 5’ AGCT 3’, cut normal DNA (assume equal amounts of each base)? (In other words, what will the average fragment size be after cutting?) Group of answer choices 256 bases 12 bases 40 bases 16 bases none of theseIf telomeres are normally shortened after each round of replication in somatic cells , what prediction would you make about the length of telomeres in Dolly, the first cloned sheep?