How many ribosomes are actively synthesizing proteins at any instant in an E. coli cell growing with a 45-min doubling time? The birth size of E. coli is 1-μm diameter and 2-μm length. The water content is 75%. About 60% of the dry material is protein, and the rate of amino acid addition per ribosome is 20 amino acids per second. The average molecular weight of free amino acids in E. coli is 126.

How many ribosomes are actively synthesizing proteins at any instant in an E. coli cell growing with a 45-min doubling time? The birth size of E. coli is 1-μm diameter and 2-μm length. The water content is 75%. About 60% of the dry material is protein, and the rate of amino acid addition per ribosome is 20 amino acids per second. The average molecular weight of free amino acids in E. coli is 126.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter15: Genes And Proteins

Section: Chapter Questions

Problem 3VCQ: Figure 15.16 Many antibiotics inhibit bacterial protein synthesis. For example, tetracycline blocks...

See similar textbooks

Similar questions

What is the major differences between bacteria that use anaerobic vs aerobic respiration and the difference between the 2 processes? Common feautures in the genomic sequences that control transcription between prokaryotes and eukaryotes? Describe some processes of active transport across membranes e g facilitated exocytosis endocytosis active transport?
The BSA precursor protein is 607 amino acids long and has a molecular weight 69.324 kDa. If a eukaryotic ribosome can synthesize an amino acid chain at 2 amino acids/sec, how many seconds would it take for 107 ribosomes to produce 1 pg of BSA precursor?
Tetracycline antibiotics bind to the A site on the bacterial ribosome and prevent further translation. Where would you expect polypeptides to accumulate on tetracycline-inhibited ribosomes? Given what tetracycline does, how does this effect cause the bacterial cell to die?
Approximately how many nucleotides make up the ATP6 gene? What are the first three nucleotides listed and which amino acid are they translated to? What is the total number of amino acids in the polypeptide chain that is translated from this DNA sequence? ATP6 gene sequence: ATGAACGAAAATCTATTCACCTCTTTTACTACCCCAACAATAATAGGACTGCCTGTTGTTGTGTTAATCGTTATGTTCCCCAGCATTCTATTTCCCTCGCCTAACCGACTAATTAATAACCGCCTAGTCTCACTCCAACAATGATTAGTACAACTTACATTAAAGCAAATACTGATTACCCACAATTACAAAGGACAAACCTGGGCCCTAATACTTATGTCTCTCATTTTATTTATTGGGTCAACAAATCTGCTAGGTCTACTACCTCACTCATTTACTCCAACTACCCAATTATCAATAAACCTAGGCATAGCCATCCCCTTGTGAGCCGGCACCGTAATCACTGGATTCCGTCACAAAACTAAAGCATCCTTGGCCCACTTTCTACCACAAGGAACACCAGTCCCCTTAATCCCTATGCTCGTAATTATCGAAACTATCAGCCTTTTTATCCAGCCCGTAGCCCTAGCCGTACGACTCACAGCTAATATTACTGCAGGCCATTTATTAATACACCTAATCGGAGGAGCTGCTTTAGCCCTAACAAATATTAGTGCCCCTACTGCTTTAATTACCTTTATCATCCTCATCCTACTGACAATTCTTGAATTCGCTGTAGCTCTAATCCAAGCCTATGTTTTTACCCTACTTGTGAGCCTGTATTTACATGATAATACTTAA
The answer given in problem 31. 17 is inadequate for me, and I simply don't understand the explanation for the answer... The problem is: Ribosoms were isolated from bacteria grown in a "heavy" medium (13C and 15N) and from bacteria grown in "light" medium (12C and 14N). These 70S ribosoms were added to an in vitro system engaged in protein synthesis. An aliquot removal serveral hours later was analyzed by density-gradient centrifugation. How many bands of 70S ribosoms would you expect to see in the density gradient? Is it possible to give a more comprehensive explanation? :)
In the absence of cladosporin, explain the initiation steps in the synthesis of lysyl-tRNA synthetase enzyme or protein in the bacterial cell, with the involvement of all initiation factors, each site in the ribosome, any important conserved sequence, subunits of ribosomes, initiation codon, charged tRNA containing what amino acid, whether it requires ATP or GTP
Unlike bacterial cells, the nucleus of a eukaryotic cell is bounded by a double-layered membrane studded by complex pores. How do you think this might affect traffic between the DNA and cytoplasm of a eukaryotic cell compared to that of a prokaryotic cell?
What is single-cell protein? Explain the reasons for the removal or reduction of nucleic acid in Single Cell Protein.
Do the following events during bacterial translation occur primarily within the 30S subunit, within the 50S subunit, or at the interface between these two ribosomal subunits? A. mRNA-tRNA recognition B. Peptidyl transfer reaction C. Exit of the polypeptide from the ribosome D. Binding of initiation factors IF1, IF2, and IF3
You gathered your data from Lab 4, and found the following values: Total Activity of lysozyme in HEW: 14 units Total Activity of lysozyme in carb 1: 21 units Total amount of protein in HEW: 70 mg Total amount of protein in Carb 1: 15 mg Calculate the extent of purification of lysozyme in Carb 1. 150% 7 fold 70% 15 fold
The dimensions of prokaryotic ribosomes are approximately 14 nm by 20 nm. If ribosomes occupy 20% of the volume of a bacterial cell, calculate how many ribosomes are in a typical cell as E. coli. Assume that the shape of a ribosome is approximately that of a cylinder.
After translation, secreted proteins are transported to the cell surface using the Golgi true false Give typing answer with explanation and conclusion