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How might incubation of plates at 37°C influence the
results of the quantitative dilution and plating of the
coconut water samples?
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- How might incubating plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?Why is the incubation temperature criticalwhen using the Filter Method when testing water samples?How does incubating plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?
- Test solution Indicator thatcan be usedInitial colour ofthe solutionFinal colourof thesolution1. Lemon juice Blue litmus RedIf there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?
- When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an effective sterilizing agent? Why are the conditions required for sterilization greater for dry heat (160-180°C for 1-2hrs) than for steam (121°C for 15min)?What are the expected problems in the centrifuge in case of imbalance of samples ? *how does the diameter of the column affect the flow rate of the elution in gel filtration chromatography?