Q: How does incubating plates at 37°C influence the results of the quantitative dilution and plating of…
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: How might storage of the coconut water samples at room temperature after collection and before…
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: Why do you have to boil the agar solution before dispensing it into tubes?
A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…
Q: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium…
A: An agar plate is defined as a petri dish that will be composed of a growth medium that is solidified…
Q: What effect does decreasing the incubation temperature have on bacterial growth rate? Why?
A: Bacterial growth is proliferation of bacterium into two daughter cells, in a process called binary…
Q: why is it that psychrotrophs placed in TSA medium are incubated at 7oC?
A: Psychrophiles are organisms that can survive and reproduce in low temperatures as low as -20 degrees…
Q: What growth factor is often supplied for cultivation of fastidious bacterial pathogens?
A: A fastidious organism requires specific nutrients for growth. In microbiology, the culture medium…
Q: Does the image above represent a pure culture? Yes O No
A: Answer- No
Q: Why are the streak plates for microorganisms in coconut water incubated at 7°C?
A: The benefits of streaking for isolation is to produce isolated colonies of an organism on an agar…
Q: If you run the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…
A: Answer: HANGING DROP TECHNIQUE : It is the special type of wet mount which is made from a drop of…
Q: Why must you exercise caution when handling raw sewagesamples?
A: Answer: Introduction: Raw sewage means a water comprising excrement, wastes products like plastic,…
Q: How can disinfection of milking equipment by farmers postpone spoilage of milk in your refrigerator?
A: The process where chemicals or physical agents called disinfectants are used to eliminate the…
Q: Why after a certain period the growth rate of the culture decreases until growth stops?
A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…
Q: Why is heat an effective sterilizing agent?
A: Heat Energy : It is a form of energy which takes place by the movement of the minute particles…
Q: What is bacterial agar?
A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient media for the growth of a…
Q: What bacteria grow on both a MacConkey agar plate and Mannitol Salt agar?
A: Selective media The media is a kind of growth media which only allow the growth of a perticular…
Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…
A: UV disinfection treatment is a very rapid physical process for disinfecting or sterilizing any…
Q: How does alkaline treatment improve the quality of agar/carrageenan extracted from the seaweed…
A: Agar is a polysaccharide created from two essential additives. Agarose and agaropectin. Agarose is…
Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…
A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…
Q: What is the purpose of food irradiation, and how does it work?
A: Irradiating food kills bacteria and extends life of the food.
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: Why does turbidity in a cultured media losses its reliability at high cell concentrations?
A: turbidity is used as a measure of cell density within a culture sample.
Q: why do We need to Wait for the iron Agar to set and then Add another thin layer of 45°C Iron Agar?
A: The test that uses iron agar is the Triple Sugar Iron (TSI) Test and is used to determine the…
Q: How might incubation of plates at 37°C influence the results of the quantitative dilution and…
A: Bacterial spores can usually tolerate high-pressure treatment (HPP). Clostridium botulinum is a type…
Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…
Q: What would you expect to see if you cultured Gram-positive, lactose-fermenting bacteria on MacConkey…
A: Given: What would happen if you culture gram positive, lactose fermenting bacteria in MacConkey…
Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…
Q: what type of bacteria may cause spoilage in coconut water that has been in the fridge for more than…
A: Coconut Water Coconut water (CW) is a slightly sweet liquid made from the endosperm of coconuts.…
Q: how often must bacteria be subcultured from a broth to maintain culture viability?
A: Answer: Sub-culturing is the method of regrowth of the bacterial cells in to a freshly prepared…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: Bacteria are of many types. They can be classified based on different properties such as cell wall…
Q: How might incubating plates at 37°C influence the results of the quantitative dilution and plating…
A: Ideal conditions are essential for the growth and reproduction of organisms including bacteria.…
Q: what is usefulness of columbia CNA agar? Can it be used to test water ?
A: Columbia Agar is a highly nutritious base medium The addition of the antimicrobial agents, colistin…
Q: how could you determine if there is any contamination on your agar?
A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…
Q: If my cultures are suffering severely due to alkaloid production and accumulation in the medium. In…
A: Alkaloids are basically the natural substances that can be used largely for human medication.…
Q: Colonies that produce alkaline waste on Hektoen enteric agar will turn O blue-green. O pink. O…
A: Colonies that produce alkaline waste on Hektoen enteric agar will turn blue- green.
Q: Why did we add agar after we measure the pH?
A: pH means p stands for potential or power.H stands for Hydrogen atom.It is the power of the Hydrogen…
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: Why does Propionigenium modestum require sodium for growth?
A: Propionigenium modestum grows from the fermentation of succinate to propionate and CO2.
Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…
Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution to a sterile petri…
A: Total dilution can be calculated by multiplication of all dilutions. Given, two dilutions are…
what might be the consequences of storaging coconut water at room temperature after collection and before plating then incubating it at 5ºC?
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Solved in 2 steps
- What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature higher than 45 oC.Why must the poured Mueller-Hinton Agar not be too shallow?How might incubating plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?
- Why do you have to boil the agar solution before dispensing it into tubes?What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
- What effect does decreasing the incubation temperature have on bacterial growth rate? Why?When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an effective sterilizing agent? Why are the conditions required for sterilization greater for dry heat (160-180°C for 1-2hrs) than for steam (121°C for 15min)?Why are the streak plates for microorganisms in coconut water incubated at 7°C?