How might the underwinding of a B-DNA helix facilitate or stabilize the formation of Z-DNA? Strands of underwound DNA spontaneously separate and reform as Z-DNA. Underwound DNA can provide a local region of unwound DNA, which allows the B-DNA to Z-DNA shift. DNA underwinding stores free energy, which facilitates the B-DNA to Z-DNA transition. Underwinding decreases Lk, which makes Z-DNA formation more favorable. Underwinding B-DNA dehydrates the polymer, forming Z-DNA.
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- Is the entire strand of DNA template strand of DNA transcribed at one time? Explain answer?a. What role does DNA replication play in DNA repair? b. What are the steps in the replication and repair of DNA?what if a mutation resulted in the enzyme DNA polymerase III being non-functional? How would that affect DNA replication
- After DNA replication, how could cells differentiate between the old and the new strand in case there is a mismatch as a result of bad replication?a) How is the lagging strand made in DNA replication? Include important enzymes and structures. How is this different from the synthesis of the leading strand? How is the structure of mRNA suited to its function? How is the structure of tRNA suited to its function? How is the structure of the nucleus suited to its function for protein synthesis?explains how the underwinding of a B-DNA helix might facilitate or stabilize the formation of Z-DNA
- What causes the change in the ability of DNA to absorb UV light when it is denatured? options: In denatured DNA, DNA double helix is disrupted, which causes the exposure of the deoxyribose and thus increases their absorbance of UV light In denatured DNA, DNA double helix is disrupted, which causes the exposure of the phosphate groups and thus increases their absorbance of UV light. In denatured DNA, DNA double helix is disrupted, which causes the exposure of the bases and thus increases their absorbance of UV light. all of the above are correctExplain how the lagging dna strand is copied during dna replication? What are the enzymes involved?How does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.
- How is the DNA unwound at the replication fork? What effect does this have on the DNA upstream of the fork, and how does the cell deal with this effect? b. Sliding clamps are topologically-closed rings that can encircle the DNA double helix. How, then, are they able to get on and off the DNA? What purpose is served by this striking topology?Create the RNA strand to be synthesized from the DNA double strand below and explain this synthesis, including the functions of the molecules responsible for synthesis. 5’-ATCGCTTGTTCGGAA-3’ 3’-TAGCGAACAAGCCTT-5’Given the following DNA sequence: 5’-ATGCGGCCAAGGTCAGAGTGACA-3’ a) If this DNA strand represents the “Sense Strand” of DNA, what would be the RNA sequence? b) If this DNA strand represents the “Antisense Strand” of DNA, what would be the RNA Sequence? c) What would be the other strand of DNA?