Human Fbh1 helicase is important in the process of DNA replication. When a mutation occurs during the production of Fbh1, the result is a mutant Fbh1 that binds at the replication fork and prevents any helicase protein from attaching to the strand. Based on this information and the image shown, what would happen during DNA replication if this mutant helicase were present? A - Topoisomerase would unwind the DNA and an RNA primer would attach to the DNA molecule and initiate replication. The process would then stop at the blue triangle because helicase is needed to separate the strands of DNA. B - Topoisomerase would unwind the DNA, but then the process would stop at the blue triangle because helicase, the RNA primer, would not be able to attach to the DNA molecule and initiate replication. C - The process would begin at the blue triangle when topoisomerase unwinds the DNA and an RNA primer attaches to the DNA molecule and initiates replication. DNA polymerase would begin the synthesis of each side, but the process would stop because helicase would not be present to rejoin the replicated strands. D - The process could not begin at the blue triangle because helicase is needed to separate the strands of DNA and initiate the process.

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 18RQ: During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol...
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Human Fbh1 helicase is important in the process of DNA replication. When a mutation occurs during the production of Fbh1, the result is a mutant Fbh1 that binds at the replication fork and prevents any helicase protein from attaching to the strand.


Based on this information and the image shown, what would happen during DNA replication if this mutant helicase were present?

A - Topoisomerase would unwind the DNA and an RNA primer would attach to the DNA molecule and initiate replication. The process would then stop at the blue triangle because helicase is needed to separate the strands of DNA.


B - Topoisomerase would unwind the DNA, but then the process would stop at the blue triangle because helicase, the RNA primer, would not be able to attach to the DNA molecule and initiate replication.


C - The process would begin at the blue triangle when topoisomerase unwinds the DNA and an RNA primer attaches to the DNA molecule and initiates replication. DNA polymerase would begin the synthesis of each side, but the process would stop because helicase would not be present to rejoin the replicated strands.


D - The process could not begin at the blue triangle because helicase is needed to separate the strands of DNA and initiate the process.

DNA-polymerase
RNA primer
DNA-ligase
DNA primase
Lagging
3' strand
5
5
5'
3'
Leading strand
Helicase
Topoisomerase
DNA-polymerase
Single-strand
binding proteins
Transcribed Image Text:DNA-polymerase RNA primer DNA-ligase DNA primase Lagging 3' strand 5 5 5' 3' Leading strand Helicase Topoisomerase DNA-polymerase Single-strand binding proteins
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