Human saliva contains the enzyme α-amylase which hydrolyzes polysaccharide acetal linkages. A soda cracker (containing starch) was carefully ground; the crumbs were distributed evenly between two test tubes. The first test tube contained only water, while the second contained a 1% solution of α-amylase. Both mixtures were heated to 35°C for 10 minutes and filtered. The filtrates were tested with iodine solution and Benedict’s solution. Use the following results to answer the questions below. Mixture Iodine test Benedict’s Test cracker in water dark blue blue cracker in water and α-amylase red red-orange precipitate a. Was the starch from the cracker/water mixture hydrolyzed? Was the starch from the cracker in the 1% α-amylase solution hydrolyzed? b. Which cracker contained reducing sugars?
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Human saliva contains the enzyme α-amylase which hydrolyzes polysaccharide acetal linkages. A soda cracker (containing starch) was carefully ground; the crumbs were distributed evenly between two test tubes. The first test tube contained only water, while the second contained a 1% solution of α-amylase. Both mixtures were heated to 35°C for 10 minutes and filtered. The filtrates were tested with iodine solution and Benedict’s solution. Use the following results to answer the questions below.
a. Was the starch from the cracker/water mixture hydrolyzed? Was the starch from the cracker in the 1% α-amylase solution hydrolyzed? b. Which cracker contained reducing sugars? |
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