I need help to produce a standard curve with the attached worksheet. The curve/graph should be absorbance versus concentration. FuneuEpose of this exercise is to familiarize or refresh students on the execution andEunction of ultraviolet/visible (UV/vis) light absorption spectroscopy.Toward that end theCeenES will determine and record the absorption values of a set of 4 standards and use thecollected data t o determine the concentration of an unknown.Familiarize yourself with theCundamentals of UV/vis absorption spectroscopy using at least your analytical chemistry(quantitative chemistry) text.I.Background InformationThe substance used in this lab is bromocresol green (BCG; you canlook up the IUPAC name).turns green in an environment with a pH less than 3.8 and blue inan environment withbetween 3.8 and 5.4).The compound is a pH indicator whicha pH greater than 5.4 (obviously blue-greenThe hydroxyl hydrogens are lost at high pH.BrBCG haswavelength (a) of about 450nm.diluting 0.0247g of BCG (s) to 100ml, with ddH,0 (distilled, deionizedwater) and a drop of 0.5M NAOH (aq)a molar mass of 698.02g/mol and a maximum absorptionStock solution of BCG is made by(for the deep blue color).Six tubes should be produced as follows:a. Blank (8000pL ddH,0)2 b. Standard 1 (7500ui ddH,0 with 500pL stock BCG) TU bR 20A c. Standard 2 (7000uL ddH.0 with 1000uL stock BCG) 4ube DPy d. Standard 3 (6500uL ddH,0 with 1500uL stock BCG) be 35°e. Standard 4 (6000pL ddH 0 with 2000pL stock BCG) be ho00f. Unknown (8000µL unknown)II.ProcedureTurn the spectrophotometer on at least 15 minutes ahead of time.a reflection of the needle on the dial then turn the front left knob to set needle to 0%transmittance.needle on the dial to 100% transmittance.first standard tube in the set; read and record the % transmittance.standard then do the same with the unknown.using A = 2 - log (&T); where A is absorbance,Line up so you don't seePlace the blank into the sample cell.Turn the right front dial to set theReplace the blank in the sample cell with theRepeat with eachConvert the % transmittance values to absorbanceRecord the absorbance values in III below.III.Dataabsorbance versus the concentration of each standard on graph paper.fit line will be ePlot (using appropriate scaling, with titles, axis labels, and units where applicable) theThe slope of the best-extinctionin A = ebc (Beer'swhere A is absorbance,coefficient, b the path length, and c the concentration of the sample).Law,theThe value of bistypically 1, so it can be ignored.1.Report the concentration of the stock BCG solution 353.2.Report the concentration of each standard tube of BCG solution.88.5b. Tube 2 H-.2.Mc. Tube 3 (ple4 uMuMa. Tube 1 22.µMd. Tube 43.Report the absorbance of each standard tube of BCG solution.0.20a. Tube 9 O.034 b. TubeTube 3 O. 7d. Tube 4Report the absorbance of the unknown BCG solution.tube4.Absorbance O.013Curve5.Produce and submit the standard curve along with this worksheet.( Makealbr i bance6.Report e for BCG and the concentration of the unknown BCG solution,5.5Concenti@tionConcentrationExtinction Coefficient of BCG O.0024

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The dissolution of the known mass of the solute in a specified volume of the solvent produces the…