i) Re-arrange the Michaelis Menten equation so it involves the ratio. Show all steps beginning Km with noting any assumptions or required conditions. d[P] = dt = K₂ [E],[S] Km+[S]' [S]O ii) Calculate the ratio for the case when the rate of product formation is 68% of Vmax and the Km substrate is in great excess. V Vmax Vmax Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient. Include in your explanation the factors that effect v and Vmax- Consider what factors make v different from or equal to Vmax. Consider what Km represents concerning processes involving ES. [S]O Km iii) Explain, in a few sentences, why the ratio determines the ratio V
i) Re-arrange the Michaelis Menten equation so it involves the ratio. Show all steps beginning Km with noting any assumptions or required conditions. d[P] = dt = K₂ [E],[S] Km+[S]' [S]O ii) Calculate the ratio for the case when the rate of product formation is 68% of Vmax and the Km substrate is in great excess. V Vmax Vmax Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient. Include in your explanation the factors that effect v and Vmax- Consider what factors make v different from or equal to Vmax. Consider what Km represents concerning processes involving ES. [S]O Km iii) Explain, in a few sentences, why the ratio determines the ratio V
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
Related questions
Question
![i) Re-arrange the Michaelis Menten equation so it involves the ratio [S]. Show all steps beginning
Km
noting any assumptions or required conditions.
Km
ii) Calculate the ratio [lo for the case when the rate of product formation is 68% of Vmax and the
substrate is in great excess.
d[P]
dt
: k₂
with =
[E],[S]
Km+[S]'
[S]o
Km
iii) Explain, in a few sentences, why the ratio determines the ratio
V
Vmax
V
Vmax
Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient.
Include in your explanation the factors that effect v and Vmax.
Consider what factors make v different from or equal to Vmax.
Consider what Km represents concerning processes involving ES.
"
iv) Calculate KM at 310K at given the following rate constant information:
k₁ = 17 s-¹M-1 at 300K with A = 7300 s-¹M-1
K-1₁ 6 s¹ at 300K with A = 14500 s -1
k₂ = 31 s¹ at 300K with A = 600 s-¹](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F424482ac-ef50-4214-a1e3-aaaeb325792a%2Ffbf398cd-cdc1-42fa-b4ab-f4fa1d8f2bf5%2Fyud82r_processed.png&w=3840&q=75)
Transcribed Image Text:i) Re-arrange the Michaelis Menten equation so it involves the ratio [S]. Show all steps beginning
Km
noting any assumptions or required conditions.
Km
ii) Calculate the ratio [lo for the case when the rate of product formation is 68% of Vmax and the
substrate is in great excess.
d[P]
dt
: k₂
with =
[E],[S]
Km+[S]'
[S]o
Km
iii) Explain, in a few sentences, why the ratio determines the ratio
V
Vmax
V
Vmax
Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient.
Include in your explanation the factors that effect v and Vmax.
Consider what factors make v different from or equal to Vmax.
Consider what Km represents concerning processes involving ES.
"
iv) Calculate KM at 310K at given the following rate constant information:
k₁ = 17 s-¹M-1 at 300K with A = 7300 s-¹M-1
K-1₁ 6 s¹ at 300K with A = 14500 s -1
k₂ = 31 s¹ at 300K with A = 600 s-¹
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