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- Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymeraseA double-stranded length of DNA is exposed to a restriction enzyme. The enzyme finds 3 recognition sites. How many fragments will be produced? a) 2 b) 3 c) 4 d) 5What is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbones
- Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme? AAGG TTCC GGCC CCGG ACCA TGGT AAAA TTTTIf a restriction enzyme cuts at this DNA sequence: TACGGAT, in general what is this sequence called?Explain how restriction enzymes recognize and digest DNA to create either blunt or sticky ends
- Which of the following sequences, in the double-stranded form, can probably be recognized by a restriction enzyme? Choose an answer from below? TTCCTT CGACGA GTCGAC GGTTGG GAGGACName the mapping technique used to determine the position of restriction sites in a DNA molecule.Which of the following is true about restriction endonucleases?a) Type I and II requires ATP to move along DNAb) Type I, II and III requires ATP to move along DNAc) Type II requires no ATP and cleaves DNA within recognition sequenced) Type II requires ATP and cleaves DNA within recognition sequence
- If a 1000 bp of DNA were inserted between the two restriction sites, how would the banding pattern on the gel differ from the one you drew in part a? (PART A WITH THE FIRST PART OF THE QUESTION IS ATTACHED)The enzyme is used to remove tension from the DNA double helix during replication called? Restriction endonuclease Gyrase DNA polymerase Helicase LigaseHow is bacterial DNA safe from digestion by Restriction Endonucleases? The plasmid of the bacteria provide antibiotic resistance Bacteriophage provide immunity to DNA degradation Foreign DNA outcompetes the chromosomal DNA Modification by methylation blocks the restriction enzyme action