Answered: If you start with TWO identical DNA…

If you start with TWO identical DNA double helices as templates, how many copies will you have after SIX rounds of PCR? 16 32 64 128 256 О

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 8SQ

See similar textbooks

Related questions

Q: Which activity of E. coli DNA polymerase I is responsible for proofreading the newly synthesized…

A: The first known polymerase is the DNA Polymerase I. It is the enzyme that participates in…

Q: To amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the…

A: PCR is the technique in which DNA molecules are replicated outside the cell, that is, in in-vitro…

Q: Which of the following ingredients does not belong in a sequencing reaction? (A ddNTPs B Primer C…

Q: After one round of PCR, one molecule of DNA consisting of two complementary strands yields how many…

A: P.C.R.--It is a short form of Polymerase Chain Reaction and common tool used in early stages of…

Q: Which DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides?…

A: DNA polymerase is the enzyme that catalyses the replication of DNA. DNA polymerase cannot synthesise…

Q: Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine…

A: Components required for PCR include a DNA sample which acts as a template , DNA primers, free…

Q: If a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: The number of DNA molecules produced after 30 cycles of PCR, starting with a single molecule, is…

A: Polymerase Chain Reaction (PCR) is a gene or DNA amplifying instrument widely used in biotechnology.…

Q: How many cycles of PCR are needed to obtain 512 amplicons? O 7 0 9 O 6

A: PCR is a revolutionary approach developed by Kary Mullis in the 1980s. PCR is based on the use of…

Q: The original DNA template starts with T following the primer sequence if the smallest fragment…

A: Dideoxy nucleotides are similar to regular, or deoxy, nucleotides, but they lack a 3’ OH group.…

Q: You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to…

A: A reaction mixture is a combination of 2 or more reactants in definite proportions to carry out a…

Q: Choose the basic chemical materials that are required to perform PCR. A. Two oligonucleotide…

A: Requirement for PCR: 2 primers: one is forward and one is reverse. Forward primer bind to…

Q: PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to…

A: Introduction The polymerase chain reaction (PCR) is an in vitro technique for amplifying a…

Q: Which of the following ingredients does not belong in a PCR reaction? DNA polymerase B) Primer…

A: Polymerase chain reaction is a method used to produce millions of copies of any DNA sample and hence…

Q: Which of the following statements is false?a) BAC is a circular DNA moleculeb) YAC is a linear DNA…

A: Cloning vectors must be a DNA sequence with low molecular weight and must be able to multiply…

Q: Which of the following ingredients does not belong in a sequencing reaction? ddNTPs Primer © Ligase…

A: Sequencing of DNA The technique to know the exact order of base pairs (nucleotide) in a DNA.

Q: R. Alu 100 100 300 F ----- 100 100 Where would you expect to see a band or bands if you used PCR to…

A: 500 bp template was prepared by using amplified PCR with forward and reverse primers of 100 bp each…

Q: After 4 cycles of PCR, how many copies of DNA would be expected, if the original number of copies…

A: PCR which is also called as Polymerase Chain Reaction is a phenomenon in which millions and…

Q: What gives the DNA molecule a negative charge? nucleobases deoxyribose ribose phosphate…

A: All living organisms are made up of cells, which are the most basic unit of life. They are…

Q: In a PCR reaction, a few seconds at high temperature disrupts the ____ that hold the two strands of…

A: Denaturation is the phenomenon of loss of helical structure of DNA. The two polynucleotide strands…

Q: Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt…

A: Introduction :- A given DNA segment can be quickly multiplied (amplified) into millions or billions…

Q: non-coding DNAs can be put into two groups

A: Part of DNA that does not encode a protein sequence is called as non coding DNA. tRNA, rRNA and…

Q: If you were starting with 25 molecules of DNA in a PCR reaction, ho much DNA would you expect to…

A: INTRODUCTION The total number of copies of double stranded DNA may be calculated using the…

Q: Your colleague made the table below comparing DNA Polymerase to RNA Polymerases. Which row is…

A: Transcription is the molecular process which involves the DNA sequences that are transcribed as…

Q: After one round of PCR, one molecule of DNA consisting of two complimentary strands yields _____…

A: The polymerase chain reaction is a technique that helps to amplify the target DNA strand. The target…

Q: DNA polymerase III adds a nucleotide to the 3' end of the strand. leading strand. All of the answers…

A: Concept used: DNA Replication DNA Replication : The process where the strands of DNA are…

Q: PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3'…

A: Introduction A primer is a single-stranded nucleic acid that all living organisms utilise to start…

Q: Sticky ends are 1. DNA fragment with single - stranded ends 2. produced by the action of DNA…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid in which…

Q: Which of the following ingredients precisely controls which small section of a very large DNA…

A: The acronym PCR stands for polymerase chain reaction. It's a test that looks for genetic material…

Q: Suppose that you are given a short fragment of DNA to sequence. You amplify the fragment with PCR…

A: Gel electrophoresis refers to the separation technique that involves the separation of charged…

Q: Which enzyme can recognize an altered base in the DNA? Group of answer choices DNA Polymerase AP…

A: There are several DNA repair mechanisms are present that are- direct repair system, excision repair…

Q: C. Estimating DNA concentration - creating a 2-fold dilution series. Can you estimate how much DNA…

A: DNA Concentration Estimation -- In molecular biology ,purity and quantitation of nucleic acids is…

Q: E.coli is replicating its chromosome. If the template DNA sequence is: 3' AAA CGC GAT 5', what would…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: If you had the RNA sequence below: 5' UUUGGAG 3' and you were going to make a piece of DNA that…

A: DNA is a molecule discovered in the nucleus by Friedrich Meischer in the late 1860s, but its…

Q: The nucleotide sequence below is one half of a double stranded DNA sequence. The highlighted portion…

A: DNA molecule is the genetic material present in the animals. Genetic material is present in the…

Q: In which sequencing method, DNA is labelled and then chemically cleaved in a sequence-dependent…

A: DNA sequencing refers to decoding the base sequence of the DNA strand. It tells us the base…

Q: What enzyme replaces the RNA primers on the Okazaki fragments on the lagging strand? DNA Pol II

A: DNA replication is the process by which DNA duplicates itself. One of the most important feature of…

Q: What causes microsatellites to be polymorphic?

A: Microsatellites are classified as variable number tandem repeats. These are 2-13 nucleotide long…

Q: A researcher combines a variety of molecules needed for DNA replication. After adding DNA to the…

A: The correct option is (A) DNA ligase

Q: Starting with one double-stranded DNA, what is the total number of double-stranded DNA after 5…

A: PCR (polymerase chain reaction):- it is a mechanism to amplify the segment of DNA into millions or…

Q: If a restriction endonuclease recognizes and cleaves a linear piece of DNA at 5 distinct places, how…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: Use the set of gene sequencing results below to answer the question that follows: A G C T -Wells I…

A: Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins…

Q: Figure 3: BestrictriOR site map showing the following: A) linear DNA that is not cut as reference B)…

A: Restriction enzymes cut the DNA (deoxyribonucleic acid) at specific sites. These enzymes recognize…

Q: An enzyme to add nucleotides specifically at the ends of chromosomes would be: Question 6 options:…

A: Chromosomes are the carrier of hereditary DNA molecules and have a thread-like appearance. They are…

Q: Which of the following is NOT a function of DNA polymerase I in E. coli? Question 1 options: A) 5'…

A: Answer: C) Helicase

Q: Once the strands have been replicated, the enzyme DNA polymerase I “cleans up” by removing the…

A: In molecular biology,DNA replication is the process in which DNA is replicated. The replicated DNA…

Q: If you need to make a lot up to 1 billion copies of a piece of DNA you would use 1. gel…

A: Polymerase chain reaction is the method of making millions of copies of a specific DNA. It was…

Q: What purpose do short DNA primers serve in PCR? They determine which sequence of DNA is copied They…

A: A known short DNA sequence is called DNA primer. It is a short nucleic acid sequence that provides a…

Question

#12

If you start with TWO identical DNA double helices as templates,
how many copies will you have after SIX rounds of PCR?
О 16
32
64
128
О 256

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

How many DNA molecules would exist after 30 PCR cycles?
Assuming you started with only one copy of a DNA sequence, how many copies should be present after 15 cycles of PCR?
For what is each of the following used in PCR: primer, DNA polymerase, 94°C?
After one round of PCR, one molecule of DNA consisting of two complementary strands yields how many molecules of DNA for a total of how many strands?
For the following short sequence of double stranded DNA, design primers (just ~ 3-4 bases) and show 2 copy cycles of PCR (refer to figure 13.25) for the amplification of this sequence of DNA (so that you have 4 double stranded DNA). 5’- GGTATTGGCTACTTACTGGCATCG- 3’ 3’- CCATAACCGATGAATGACCGTAGC- 5’
How many copies of a target DNA molecule are made after 30 cycles of PCR?
After one round of PCR, one molecule of DNA consisting of two complimentary strands yields _____ molecules of DNA for a total of ______ strands
Why is it necessary to use a special DNA polymerase(Taq polymerase) in PCR?
The sequence of a template DNA is GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The sequence of a primer used for PCR amplification of the above DNA is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively? answer options: 1) Left, left 2) Right, left 3) Left, right 4) Right, right 5) Right, left
A scientist programed his PCR machine to perform five complete cycles of PCR starting with one double stranded DNA molecule, how many molecules of DNA did he get at the end assuming all conditions went normally?
All of the following are needed for PCR except: A) dNTP nucleotide monomers B) Oligonucleotide primers C) Restriction endonuclease D) Template DNA E) DNA polymerase
Which of the following is NOT a characteristic of PCR primers? Short synthetic oligonucleotide Typically 18-25 bases in length Double stranded DNA Unique homology to the DNA template Sequence with ~50% G:C content