In differential translation, a different transcript may be recovered depending on which TATA box is used. O True O False D Question 22 Heterozygous mice for the Sd gene are shown to have relatively shorter or lack a tail. True False
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- In prokaryotes, transcription and translation occursimultaneously. In eukaryotes, the nuclear membranerestricts transcription to the nucleus; mRNAs aretranslated only after transport into the _______?.true/ false Drosha and Pasha bind to mRNAs allowing the 43S subunit to form, but they inhibit the formation of the 80S subunit.Briefly explain the importance of the protein factor EF-Ts in the translation process. Do not simply define the given.
- True or false: A fully assembled 70S ribosome is recruited to the mRNA just 5 ́ of the translation start site?The chemical bisulfite transforms cytosine (C) to uracil (U) (U). In DMSO, bisulfite is dissolved. Bisulfite was treated ribosomes. The bisulfite was subsequently removed, and the amount of protein produced was determined using a translation assay. Why do you think we need a DMSO treated control group instead of a group not treated with anything?Formation of the 70S ribosomal initiation complex for prokaryotic translation requires A. Mn2+ B. EF-Tu C. tRNAMet D. tRNAfMet D. IF-2 E. GTP
- 19Eukaryotic messenger RNA and hnRNA both have poly (A) tails at their 3'-ends. Yesorno 20Messenger RNA splicing and export are uncoupled processes. Yesorno twenty oneWhich of the following plays an important role in directing newly synthesized proteins to specific cellular destinations?A.signal sequenceb.nuclear localization signalc.chain elongation stretchD.Shine – DalgarnoThe tRNA molecule carries EIF2 and GTP and binds the small ribosomal subunit at the P site in the first steps of translation initiation. tue or falseThe Events in Transcription Initiation Describe the sequence of events involved in the initiation of transcription by E. coil RNA polymerase. Include in your description those features a gene must have for proper recognition and transcription by RNA poIymerase.
- Figure 28.41 gives some examples of recombination in IgG codons 95 and 96, as specified by the Vkand Jkgenes. List the codon possibilities and the amino acids encoded if recombination occurred in codon 97. Which of these possibilities is less desirable?A _____________________ is a purine-rich sequence closeto AUG (the initiation codon) on a prokaryotic mRNA thatbinds to a complementary sequence on the 30S ribosomesubunits, thereby promoting the formation of the correctpreinitiation complex.For the following mRNA sequence (reading from left to right) what will be the amino acid sequence following translation? (Use chart provided) AUGCCAGUUGAAUAA A. Pro-Val-Met-Leu-His B. Met-Val-Tyr-Pro C. Met-Pro-Val-Glu D. Correct answer not given E. Met-His-Phe-Ala-Arg