In order to function as an oxidative phosphorylation uncoupler, 2,4-dinitrophenol must act catalytically, not stoichiometrically. What does this mean? Identify and discuss an important implication of this conclusion.
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In order to function as an oxidative phosphorylation uncoupler, 2,4-dinitrophenol must act catalytically, not stoichiometrically. What does this mean? Identify and discuss an important implication of this conclusion.
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- If you were to determine the P/O ratio for oxidation of α-ketoglutarate,you would probably include some malonate in your reaction system. Why?Under these conditions, what P/O ratio would you expect to observe?If you were to determine the P/O ratio for oxidation of a-ketoglutarate, you would probably include some malonate in your reaction system. Why? Under these conditions, what P/O ratio would you expect to observe?How can we test the validity of the mechanism proposed for the catalytic triad?
- The isoelectric point (pI) of 6-phosphogluconate dehydrogenase is 6.0. Explain why the buffer used in DEAE cellulose chromatography must have a pH greater than 6 but less than 9 in order for the enzyme to bind to the DEAE resin.Assume that the reaction Arabinose +Pi <> Arabinose-6-P has a delta G of +13.0 kJ/mol and the reaction XTP <> XDP + Pi has a delta G of -25.7 kJ/mol. Calculate the delta G for the combined reaction Arabinose + XTP <> Arabinose-6-P + ADP, in kJ/mol to four significant figures.In many biochemical reactions which involves the formation of an enolate intermediate, the carbonyl oxygen of the substrate is coordinated to a divalent metal ion (usually zinc or magnesium) in the active site. Explain with structural drawings, how this ion-dipole interactions affect the acidity of the a-protons?
- Why is triose phosphate isomerase considered to be catalytically perfect?The hydrolysis of a phenylalanine-containing peptide is catalyzed by a-chymotrypsin with the following results. Calculate KM and Vmax for the reaction. What is the equation of the line?What is the catalytic triad on chymotrypsin? Detail its importance.
- An inhibitor that specifically labels chymotrypsin at histidine 57 is N-tosylamido-l-phenylethyl chloromethylketone. How would you modify the structure of this inhibitor tolabel the active site of trypsin?UDP-glucuronosyltransferase enzymes bind the organic compound UDP-glucuronic acid (UDP-GA) in order to catalyse the transfer of a glucuronic acid group from UDP-GA to a drug molecule, releasing UDP from the active site as a product. UDP is then regenerated by the activity of another enzyme. What terms could be used to describe UDP-GA?An inhibitor that specifically labels chymotrypsin at histidine 57 is Ntosylamido-l-phenylethyl chloromethyl ketone. How would you modify the structure of this inhibitor to label the active site of trypsin?