If you were to determine the P/O ratio for oxidation of a-ketoglutarate, you would probably include some malonate in your reaction system. Why? Under these conditions, what P/O ratio would you expect to observe?
Q: Please provide a chemical explanation for why the addition of water across the double bond is…
A: Metabolism is the chemical reactions that occur in cells and organisms. It involves catabolism and…
Q: The following data were obtained in a study of an enzyme that is known to follow the…
A: Michaelis menten constant, Km is the substrate concentration required to produce half maximum…
Q: Why does biological Fe2+ oxidation under oxic conditions occurmainly at acidic pH?
A: Oxic condition refers to the process or environment in which oxygen is involved.
Q: Pyridoxal phosphate acts as an electron sink, stabilizing carbanionic intermediates. What other…
A: Pyridoxal phosphate is necessary for a variety of processes in human metabolism, most notably in the…
Q: The mechanism involved in the reaction catalyzed by phosphoglyceromutase is known to involve a…
A: Glycolysis is breakdown of glucose to provide energy. Phosphoglyceromutase catalyze the conversion…
Q: What structurtal property of mono- and polyunsaturated fatty acids prevents oxidation of these…
A: Fatty acids are important lipid molecules. They are characterized by the presence of a carboxylic…
Q: The KM for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8 × 10−2 M, and the KM…
A: The Michaelis-Menten equation, at some unique substrate concentration, defines the rate of catalysis…
Q: If you were to determine the P/O ratio for oxidation of a-ketoglutarate, you would probably include…
A: The P/O ratio or Phosphate/Oxygen ratio refers to the amount of ATP produced from the movement of…
Q: Using the half reactions from the given table, what is the reaction of this reduction-oxidation?
A: Tricarboxylic acid cycle is the final common oxidative pathway for oxidation of carbs, fats and…
Q: The turnover number of the enzyme fumarase that catalyzes the reaction,
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: If you were to determine the P/O ratio for oxidation of α-ketoglutarate,you would probably include…
A: The P/O ratio or Phosphate/Oxygen ratio refers to the amount of ATP produced from the movement of…
Q: Explain how the conversion of alanine to pyruvic acid (pyruvate) can be identified as an oxidation…
A: Oxidation is the gain of oxygen, loss of electrons, and loss of hydrogen from an atom. The oxidation…
Q: Beginning with the 1st tetrahedral intermediate, show the complete steps in chymotrypsin mechanism…
A: The peptides are formed by linking different amino acids through peptide bonds. The enzyme involved…
Q: What impact does temperature have on the activity of -Amylase?
A: α-Amylase is an enzyme that is known to catalyze starch hydrolysis.
Q: In order to function as an oxidative phosphorylation uncoupler, 2,4-dinitrophenol must act…
A: An uncoupler is a molecule that disrupts oxidative phosphorylation in prokaryotes and mitochondria…
Q: The glycine cleavage system is a group of four enzymes that together catalyze the following…
A: Glycine is a simple non-essential amino acid. Glycine is required for the synthesis of glucose and…
Q: The metabolism of alcohol pathway is showed in the figure characterized by biotransformation of:…
A: Metabolism- The primary enzyme involved in the metabolism of alcohol is ADH( Alcohol dehydrogenase)…
Q: [methyl-14C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate…
A: Succinate dehydrogenase or respiratory complex II is an enzyme complex, found in many bacterial…
Q: Iodoacetate is a potent inhibitor of glyceraldehyde-3-phosphate dehydrogenase. What chemical…
A: Iodoacetate is an inhibitor of glyceraldehyde-3-phosphate dehydrogenase(GADPH) , a glycolytic enzyme…
Q: Which of the following redox reactions would be expected to proceed as written? (Assume standard…
A: Gibbs free energy change is the thermodynamic potential that can be used to calculate maximum…
Q: N-Acetylglutamate is required as a cofactor in the synthesis of carbamoyl phosphate. How is…
A: Cofactors are non-protein part of the enzymes that helps in the catalytic activity during a…
Q: Show the carbon atom that changes oxidation state during the reaction catalyzed by…
A: Glyceraldehyde-3-phosphate dehydrogenase is an enzyme that catalyzes the sixth step of glycolysis in…
Q: Are any of the intermediates in the β-oxidation pathway chiral? Explain.
A: Chiral: it is defined as a molecule with lack of internal plane of symmetry and has four different…
Q: Given each set of information which may include common name(s) and the reaction catalyzed, you are…
A: Note : Hi ! Thank you for the question. We are authorized to answer three subparts at a time. Since…
Q: Pyridoxal phosphate acts as an intermediate carrier of amino groups during transamination reactions.…
A: Pyridoxal phosphate is the active form of Vit B6. It is a coenzyme in a variety of enzymatic…
Q: How many moles of ATP are synthesized by adding stearate to TCA through B-oxidation? Why is that?…
A: From the Beta oxidation process: The P/O ratios, which give the number of ATP produced per molecule,…
Q: How many tritium atoms (3H) are incorporated into palmitate when fattyacid synthesis is carried out…
A: Palmitate synthesis requires the input of 8 acetyl CoA molecules, 14 NADPH molecules and 7 ATPs.…
Q: Given each set of information which may include common name(s) and the reaction catalyzed, you are…
A: Enzymes have a common name that provides little information about the reaction catalyzed by…
Q: If you were to determine the P/O ratio for oxidation of a-ketoglu- tarate, you would probably…
A: During the oxidation of α-ketoglutarate, some malonate may be added to block succinate…
Q: activity of -α Amylase?
A: Effects of temperature on enzymes activity: The amylase enzyme breaks down starch fast at the right…
Q: Determine the catalytic power of jack bean urease in catalyzing the hydrolysis of urea given the…
A: ENZYMES : - Enzymes are defined as the organic molecules which helps in catalyzing the reaction.…
Q: Ascorbic acid: What does it mean to say that ascorbic acid is a strong reducing agent? What is the…
A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: What is the rationale in using hexokinase and G6PD in the in the reverse reaction of Oliver-Rosalki?
A: Oliver rosalki method: The method of analysis of creatine kinase by measuring the Absorbance of NADH…
Q: Intestinal microbes produce butyrate, which helps regulate the immune system. How many rounds of β…
A: Microorganisms or microbes are microscopic organisms that exist as unicellular, multicellular, or…
Q: Why is citrate an appropriate inhibitor of phosphofructokinase?
A: Citrate allosterically inhibits phosphofructokinase by enhancing the inhibitory effect of ATP
Q: The isoelectric point (pI) of 6-phosphogluconate dehydrogenase is 6.0. Explain why the buffer used…
A: Enzymes are a class of proteins except for ribozymes (class of RNA) that elevates the pace of the…
Q: An L-amino acid oxidase in mammalian peroxisomes requires H2O and O2 as substrates and catalyzes…
A: L-amino acid oxidase are flavoenzymes.
Q: Determine whether the following reactions will be spontaneous under standard biochemical conditions.…
A: An exergonic reaction is one in which the energy level of the products is lower than the energy…
Q: Mevalonate is a component of which biochemical pathway?
A: The carboxylate anion of mevalonic acid is known as mevalonate is of major pharmaceutical…
Q: In the hexokinase reaction (in glycolysis) Keq caclualtion, what is the OBVIOUS problem with getting…
A: Cellular respiration is a catabolic pathway of the process of metabolism, where a series of chemical…
Q: Is Glu-97 directly involved in reaction with the substrate (substrate = DHAP, enzyme = TIM)? What…
A: In case of enzyme TIM (triosephosphate isomerase), Glu-97 remain present adjacent to the active site…
Q: Which of the following reactions would you expect to proceed in the direction shown, under standard…
A: The correct reactions are : (a), (b), (c).
Q: . Using the principles described in the text regarding pyridoxal phos- phate mechanisms, propose a…
A: Introduction- Serine hydroxy methyltransferase (SHMT) is known as a pyridoxal phosphate-dependent…
Q: Why is triose phosphate isomerase considered to be catalytically perfect?
A: Diffusion-limited reactions are reactions where the rate of the reaction is solely dependent upon…
Q: Pyridoxal phosphate stabilizes carbanionic intermediates by serving as an electron sink. Which other…
A: Pyridoxal phosphate is required for different reactions in human metabolism, primarily in the…
Q: In some brain cancer cells, a mutated form of isocitrate dehydrogenase, instead of catalyzing the…
A: The brain is part of the central nervous system. It is located inside the skull. The brain has three…
Q: Suggest a name for an enzyme that catalyzes each of the following reactions. Oxidation of nitrite…
A: The catalysis in which the performance of the biological molecule increases by the enzyme is called…
If you were to determine the P/O ratio for oxidation of a-ketoglutarate, you would probably include some malonate in your reaction system. Why? Under these conditions, what P/O ratio would you expect to observe?
Step by step
Solved in 2 steps
- Examine the ActiveModel for alcohol dehydrogenase and describe the structure and function of the catalytic zinc center.If you were to determine the P/O ratio for oxidation of α-ketoglutarate,you would probably include some malonate in your reaction system. Why?Under these conditions, what P/O ratio would you expect to observe?Intestinal microbes produce butyrate, which helps regulate the immune system. How many rounds of β oxidation of palmitate are required to produce butyrate?
- In order to function as an oxidative phosphorylation uncoupler, 2,4-dinitrophenol must act catalytically, not stoichiometrically. What does this mean? Identify and discuss an important implication of this conclusion.In some brain cancer cells, a mutated form of isocitrate dehydrogenase, instead of catalyzing the oxidation of the secondary alcohol of isocitrate, catalyzes the reduction of a-ketoglutarate. Draw the product of the reaction.With respect to stereochemistry, what is unique about the reaction catalyzed by aconitase?
- What are the possivble oxidation product of catalase using H2O2 as the substrate? Explain in 1-3 sentencesThe isoelectric point (pI) of 6-phosphogluconate dehydrogenase is 6.0. Explain why the buffer used in DEAE cellulose chromatography must have a pH greater than 6 but less than 9 in order for the enzyme to bind to the DEAE resin.In the hexokinase reaction (in glycolysis) Keq caclualtion, what is the OBVIOUS problem with getting a Keq of 678? what should keq be normally? how can you EXPLAIN a Keq of 678?