If you were to determine the P/O ratio for oxidation of a-ketoglu- tarate, you would probably include some malonate in your reaction system. Why? Under these conditions, what P/O ratio would you expect to observe?
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During the oxidation of -ketoglutarate, some malonate may be added to block succinate dehydrogenase. This would help to note down the phosphorylation that occurs only from -ketoglutarate dehydrogenase alone.
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- Examine the ActiveModel for alcohol dehydrogenase and describe the structure and function of the catalytic zinc center.Using the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.The Reactions and Meehanisms of the Leloir Pathway Write the reactions that permit galactose to be utilized in glycolysis. Write a suitable mechanism, tor one of these reactions.
- Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.If you were to determine the P/O ratio for oxidation of a-ketoglutarate, you would probably include some malonate in your reaction system. Why? Under these conditions, what P/O ratio would you expect to observe?If you were to determine the P/O ratio for oxidation of α-ketoglutarate,you would probably include some malonate in your reaction system. Why?Under these conditions, what P/O ratio would you expect to observe?
- Intestinal microbes produce butyrate, which helps regulate the immune system. How many rounds of β oxidation of palmitate are required to produce butyrate?Are any of the intermediates in the β-oxidation pathway chiral? Explain.How many tritium atoms (3H) are incorporated into palmitate when fattyacid synthesis is carried out in vitro with the following labeled substrate?
- In the hexokinase reaction (in glycolysis) Keq caclualtion, what is the OBVIOUS problem with getting a Keq of 678? what should keq be normally? how can you EXPLAIN a Keq of 678?What is the catalytic triad on chymotrypsin? Detail its importance.With the help of the half reactions given in Table 1, formulate the redox equation for the oxidation of succinate and reduction of ubiquinone.