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- Why do some scientists use immersion fluids when looking at their microscope slides? Increases the working distance Keeps the sample alive Collects a narrower band of light Takes advantage of lower refractive index Increases the numerical apertureThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?Which of the following factors affect the specific rotation of an optically active substance? Question 1 options: Length of the sample tube Concentration of the substance Temperature Wavelength of the light All of these
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceYou should always position the cells or tissues in the center of view before increasing magnification, because... You know that the image will move right as you move the slide to the left. You know that the image will move up as you move the slide down. You know that the center of the field of view will be unchanged. This is the best way to avoid breaking the slide with an objectiveWhich of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.
- Why are stereomicroscopes often referred to as dissecting microscopes? Stereomicroscopes come apart (dissect) easily. The large distance between the lens and the specimen makes them ideal for dissections. They are good for viewing much smaller objects. Only a few specimens will fit under the stereomicroscope. The two eyepiece lenses on a stereomicroscope give a better perception of depth.Match the following items: [4] 1. 540 nm A. Wavescan 2. Quantity of light of a specific wavelength absorbed by a sample B. Standard series 3. Reference samples with a concentration range C. Absorbance 4. Absorbance values at all wavelengths D. PurpleRadiology technicians in a hospital setting expose hundreds of images every day. It is the responsibility of the radiologist to review these films daily. The department uses digital imaging, in which the radiologists can view the films on monitors that allow them to manipulate the image without having to expose the patient again if the film is too dark or too light. What is the term for when the radiologists can review the images on a monitor?
- It is desirable that the microscope objectives be parfocal because the specimen will be .................. a)blurry due to the changes in the refractive index of the medium b)stay in focus when the light is turned off c)have more contrast and enhanced details d)remain in focus if the magnification is changed e)none of the answer choicesAll other things being equal, the objective lens with the highest value for numerical aperture (N.A.) will always have the lowest______? A. Field Of View B. Magnification C. Image Quality D. Limit Of ResolutionA student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometers