2. What is the function of a revolvillg3. Which connects the eyepiece to the revolving nosepiece with the objectives?4. Describe the function of the mirror.5 What are parts of the microscope are being connected by the inclination joint?What I Have LearnedDirections: Fill in the blank to make the sentences complete. Choose the correct words listedin the box.is a curved glass that bends light that passes through it.regulates the amount of light on the specimen.1,2.for viewing.3. The glass slide is placed on the4. The bottom of the microscope, used for support is5. The6.7. To transfer a microscope, use theholds two or more objective lenses and can be rotated to easily change power.is the lens at the top of the microscope that you look through.to carry the microscope.8.is a small knob on the side of the microscope used to fine-tune the focus of thespecimen.9. The arm and base of themicroscope are being connected by the10. Theis used to enlarge the specimen.stageeyepieceobjective lensrevolving nosepiecearmbasemirrordiaphragminclination jointfine adjustment knob

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Fundamentals of Sectional Anatomy: An Imaging Approach

2nd Edition

ISBN:9781133960867

Author:Denise L. Lazo

Publisher:Denise L. Lazo

Chapter3: Face

Section: Chapter Questions

Problem 10RQ

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5. Which objective lens provides the greatest field of view? a. high. b. 100X c. low d. This depends on if the specimen is stained
1. What happens if you try to use the coarse adjustment when the 10X lens is in place? 2. Assuming the light is on and the oculars are in place, what are the next 2 steps? (Answer in 3-5 sentences)
1. What three things change as you increase magnification? 2. How do you properly use a microscope?
1. after going through all 4 objectives, which would give you the largest field of view? greatest depth of focus? why? 2. how do you calculate the total magnification of a specimen? show sample
1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…
1. You're using a microscope with 10x magnification ocular lenses and a 25x magnification objective lens. What is the microscope's overall magnification at this setting? 2. Is it correct that immersion oil improves image magnification and resolution?
Which of the follow are correct statements about microscope handling and care? 1. Always use TWO HANDS to carry the microscope.  2. Only use LENS PAPER to clean the lenses. Never wipe back and forth, but simply make a gentle swipe across the lens. 3. A soft cloth should be used to clean the lenses. 4. Start and finish the day’s work at the LOWEST MAGNIFICATION. 5. Start and finish the day’s work at the HIGHEST MAGNIFICATION 6. Always BEGIN examining a specimen with a LOW POWER objective. 7. Always BEGIN examining a specimen with a HIGHEST POWER objective 8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective. 9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective. 10. Unplug the microscope by pulling on the plug, not the cord.
The girl is focusing a slide and she is moving the stage up toward the slide. a) What has happened to the slide? b) Is this the proper way to focus? c) Should you focus by moving stage up or down? d) What happens to a microscope i' place at the edge? e) What should you do to prevent this from happening.
Place the path of light (using the numbers) in the microscope in the proper order as the light travels from its source until it hits the researcher’s eye: 1) condenser 2) light source 3) nose- piece lens 4) ocular lens 5) slide a) 1, 2, 5, 4, 3 b) 1, 3, 4, 2, 5 c) 2, 1, 5, 3, 4 d) 3, 2, 1, 4, 5 e) 1, 3, 4, 2, 5
Instruction: FILL IN THE BLANKS 1. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray. 2. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed. 3. In shifting to higher magnification, simply rotate the _________________ to the desired objective. If the image is not clear manipulate the __________________ knob. 4. The total magnification of the specimen as seen in the microscope is 400x. This means that the ocular magnification is _______ x and the ______________ is 40x.
Answer all the questions. 1. How do you calculate total magnification? 2. Which lens requires the use of cedarwood oil? Why is it important to use oil when focusing under this lens?

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