In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different? b) What is “coating buffer” for an ELISA? Does the buffer molecule used make sense based on the desired pH? In an ELISA procedure the samples are incubated and the ELISA plate is covered with parafilm and placed in a humidified chamber to prevent evaporation of the small liquid volumes in the wells. c) How would your results change if you did not incubate in a humidified chamber?

In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different? b) What is “coating buffer” for an ELISA? Does the buffer molecule used make sense based on the desired pH? In an ELISA procedure the samples are incubated and the ELISA plate is covered with parafilm and placed in a humidified chamber to prevent evaporation of the small liquid volumes in the wells. c) How would your results change if you did not incubate in a humidified chamber?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 4RQ

See similar textbooks