The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?

Q: Why is it important to limit the quantity of cells used to prepare a smear?

A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…

A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Why does a streaking method used to inoculate plates result in isolated colonies?

A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…

Q: What is the difference between culture-based technique and culture-independent techniques based on…

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: FNA

A: The above test is called the COAGULASE test. The coagulase test is a experiment to determine whether…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?

A: Answer:  Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…

A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…

Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…

A: Depending on their composition, media can be classified as defined and undefined media. Defined…

Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

A: The  bacterial species are responsible for causing several diseases within the human body. So, for…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: You were given a mixed nutrient agar broth culture of bacteria  1a. How will you determine the…

A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…

Q: Explain why it is important to use only a small amount of bacteria when preparing a smear

A:

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: Which of the media used in these labs (MSA, BAP, CAP) was selective? differential? highly enriched?

A: INTRODUCTION Differential media Used to differentiate among organisms Selective media Selectively…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.

A:

Q: What is the purpose of adding sodium bicarbonate to a culture medium?

A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…

Q: Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the…

A: In the laboratory blood samples are used to detect various disease conditions. There are different…

Q: Before using the API® Staph System, what tests should be performed on bacteria to confirm that they…

A: API Staph (analytical profile index) is a systematized procedure used for the identification of the…

Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…

A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…

Q: What is the coliform test when is it used? What is its purpose? How does it function? What are the…

A: All water that is naturally exposed to the atmosphere, such as rivers, lakes, and reservoirs. It is…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…

A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…

Q: in an indirect elisa procedure what enzyme is used?

A: Enzyme-linked immunosorbent assay (ELISA) is an assay technique used for detecting and quantifying…

Q: how to find out the test is differential or selective in the microbiology lab test?

A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…

Q: Explain with the examples of biochemical test that are used to identify the bacteria in the…

A: The smallest organism that is usually defined that the are been can be seen with the naked eye is…

Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…

A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…

Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…

A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…

Q: Why are plated media stored in an inverted position?

A: Plated media enables the isolation of microorganisms from samples or specimens.

Q: Name three reasons why the ELISA test is useful in detecting microbes

A: ELISA:            ELISA ~ Enzyme-Linked Immunosorbent Assay                   A screening tool used…

Q: In the conduct of serial dilution, which of the following tools is NOT necessary?   a. Agar…

A:

Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…

A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.