In the isolation of DNA which of the following best describe the purpose of using NaCl-SDS solution?
Q: what are advantages and disadvantages of using STRmix software in degraded DNA and DNA mixtures?
A:
Q: In assessing extracted DNA quality, what does A280 indicate? aromatic amino acids nitrogenous bases…
A: DNA is extracted from specific cells to study the extracted DNA. The purity of extracted DNA is…
Q: Explain in lengthy the principle of electrophoresis in nucleic acid isolation. Explain in detail the…
A: 1)Nucleic acid electrophoresis Gel electrophoresis is scientific technique used which helps us…
Q: Alcohol is considered to be a significant reagent for the isolation of nucleicacids (RNA and DNA).…
A: In molecular biology, the extraction of nucleic acids DNA and RNA is the most crucial method. DNA,…
Q: In DNA extraction what else might be in the ethanol/aqueous interface? How could you eliminate it
A: Monovalent cations and ethanol play a key role in removing the solvation shell that surrounds DNA,…
Q: What are the three general steps involved in the isolation of proteins? Discuss or briefly describe…
A: Proteins are biomolecules made up of one or more long chains of amino acids. Proteins catalyze…
Q: In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?
A: DNA is extracted from different sources to analyze and study the DNA sequence and diseases caused…
Q: When proteins are separated using native gel electrophoresis, size, shape, and charge control their…
A: Gel electrophoresis is a method for separation and analysis of macromolecules like DNA, RNA, and…
Q: When determining the purity of DNA, it understood that pure DNA is identified by a ratio of between…
A: When isolating nucleic acids from a cell, the final product is a mixture of DNA, RNA and Proteins.…
Q: Which of the following is NOT used in an in situ hybridization experiment for visualizing DNA? (CSLŐ…
A: Nucleic acid hybridization: It is a fundamental tool in molecular biology used to identify the…
Q: Provide detailed explanation of the following super-resolution techniques: GSD, RESOLFT
A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…
Q: What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…
Q: What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative…
A: DNA stands for deoxyribonucleic acid, that is a molecule that contains the directions an organism…
Q: Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein)…
A: Gel Electrophoresis It is a technique which is used to separate the fragment of macromolecules like…
Q: The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily…
A: How size determine the rate of migration:- Smaller DNA molecules migrate Faster through the gel.…
Q: What are the importance of accurate wavelength measurement in purified DNA sample?
A: DNA is a double helical hereditary molecule. The nucleosome is a monomer of the DNA. In DNA, four…
Q: Briefly explain in situ hybridization, giving some applications of this technique.
A: Introduction Molecular biology deals with various techniques for study of structure and functioning…
Q: Which well(A through E) of this agarose gel contains the smallest DNA size? Please look at the pic.
A: Gel electrophoresis technique of separation of molecules on the basis of their size and charged…
Q: Can DNA be isolated from beef? Discuss the process briefly
A: The purification of DNA from the collected sample of DNA with the help of the various methods and…
Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…
A: There are different molecular biology tools and techniques used to analyze and quantify…
Q: Explain the importance of adding TE Buffer as the final solution to isolate the concentrated DNA.
A: DNA isolation is a process of extracting nuclear material from a tissue sample. The tissue sample is…
Q: IT IS POSSIBLE TO ESTIMATE THE CONCENTRATION OF SPECIFIC DNA SEQUENCE IN A SOLUTION OF MIXED DNA.…
A: DNA can be quantified through nanodrop with a particular absorbance.
Q: If DNA is extracted from the juvenile leaves of mint plant. Then how to check the quantity and…
A: Gel electrophoresis is a technique used widely to separate the DNA molecule based on its charge and…
Q: In DNA and plasmid extraction using the kit method, silica membrane is used. How could the nucleic…
A: Silica based DNA extraction method is an easy and economical method for extracting nucleic acid. It…
Q: In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of…
A: DNA (Deoxyribonucleic acid) is a double helical structure that consists of nucleotide. Nucleotide…
Q: If we have different protein samples. How would we analyze them using SDS- PAGE and 2-D gel…
A: Purification of proteins is a critical step in gaining a full understanding of the protein's…
Q: Explain the rationale behind the separation of different cellular components using centrifuge.
A: centrifugation works on the basis of sedimentation rate and density of the cellular components.…
Q: For the isolation of DNA from buccal cells using saline solution, concentrated soap, and ice cold…
A: For isolating DNA from buccal cells. First, we need buccal cells which we are getting…
Q: In ion torrent DNA sequencing, covalent bond formation is detected by an ion sensor sensitive to…
A: It's a DNA sequencing method
Q: What does A260 indicate, in assessing extracted DNA quality? A carry-over salts B nitrogenous bases…
A: A260 is the absorbance measured at 260 nm at which the nucleic acids DNA and RNA absorb light.
Q: What is used to identify the size of a DNA molecule during gel electrophoresis?
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Q: What is the role of alcohol in extracting DNA? O DNA is a polar molecule with an overall negative…
A: DNA is deoxyribonucleic acid, is a nucleic acid it is deoxy in the 2nd Carbon that makes DNA more…
Q: Explain how DNA replication is used to make base sequencing possible with Sanger's method and the…
A: DNA sequencing is done to find out the order of nucleotides along a DNA strand.The chemical building…
Q: Explain how DNA replication is used to make base sequencing possible with Sanger’s method and the…
A: Sequencing is the determination of the order of nucleotides bases in a DNA (deoxyribonucleic acid)…
Q: During agarose gel electrophoresis, why does DNA move through the gel when electric current is…
A: Gel electrophoresis is basically a method that aid in separating DNA fragments and other molecules…
Q: the following correctly describes a possible scenario dunng a run of gel electrophoresis wit DNA…
A: This is a technique widely used for the separation of DNA fragments. These are isolated according to…
Q: What compounds remain in the ethanol solution after the DNA is removed bt spooling onto a glass rod?
A: Introduction: The extraction of DNA is the first step for subsequent molecular or forensic…
Q: What is the function of each reagent used in the extraction of DNA from banana? •Saltwater…
A: The extraction of DNA from any cell involves the following steps: Homogenization of the cells to…
Q: Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of…
A: Given- Stock solution of DNA sample- 0.1 mg/ml Volume required= 2 ml Concentration of the DNA stock…
Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to
A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…
Q: Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of…
A: Gel electrophoresis is a technique which is used to separate the molecules under the application of…
Q: Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay…
A: This assay is used to measure the concentration of protein.
Q: Once you had your DNA sample extracted from your cells, why did we add the yellow Master Mix…
A: The DNA sample extracted from the cells is amplified using a method called the Polymerase Chain…
Q: Explain why DNA fragments migrate in a gel electrophoresis. Which fragments migrate farthest: large…
A: Introduction :- The technique of gel electrophoresis is used to separate DNA fragments based on…
Q: Why the protien contamination happens during DNA isolation how the isolated DNA get contaminated by…
A: During the cycle of DNA isolation, the DNA test more often get contaminated with RNA and protein. To…
Q: What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?
A: Fluorescent labeling and radioactive labeling have found wide applications in DNA sequence analysis…
Q: Explain the purposes behind replica plating and the Ames test.
A: Replica plating is a microbiological technique in which one or more secondary Petri plates…
Q: What are the different methods of quantifying DNA? How would you determine if your
A: Quantitation of DNA ,methods used for quantifying DNA ---* Quantifying of DNA -- In various methods…
Q: Define Buoyant density. How can you isolate genomic and plasmid DNA from E.coli using with this…
A: Buoyant density is defined as the density of a substance or molecule at which it attains equilibrium…
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- What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments? GelRed® moves down the agarose gel in response to the electric current and enables visualisation of the position of the nucleic acids within in the agarose gel. GelRed® intercalates with the Nucleic acid and, under UV light, fluoresces to enable visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the Nucleic acid and enables visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the amino acids in the agarose gel and enables visualisation of the position of their in the agarose gel.
- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityIf you have G-C rich DNA versus A-T rich DNA. Will the melting temperature be the same? Why? Will the annealing temperature be the same? Which of the categories in (a) above is more prone to errors?Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either pole but in fact moves by diffusion through the TAE buffer in the agarose. b. DNA can be attracted to either pole depending on the bases it is made of. c. DNA is attracted to the negative pole. d. DNA is attracted to the postive pole
- DNA solution is viscous because of the nature of chemical substance that can intercalate into the DNA helix. An example of such substance is acridine orange. experiments revealed that acridine orange causes an increase in the viscosity of DNA solution.how would you account for this effect?The complementarity of its two strands is the underlying reason that DNA can be faithfully copied. Propose alternative chemical structures that could be faithfully copied.Suppose you have subjected the two given samples of EcoRl digested DNA to get electrophoresis. Draw a diagram of the expected gel to show the location of the cut DNA pieces. 
- A solution contains DNA polymerase and the Mg ²+ salts of dATP, dGTP, dCTP, and TTP. The following DNA molecules are added to aliquots of this solution. Which of them would lead to DNA synthesis? (a) A single-stranded closed circle containing 1000 nucleotide units. (b) A double-stranded closed circle containing 1000 nucleotide pairs. (c) A single-stranded closed circle of 1000 nucleotides base-paired to a linear strand of 500 nucleotides with a free 3' -OH terminus. (d) A double-stranded linear molecule of 1000 nucleotide pairs with a free 3’-OH group at each end.What is the role of alcohol in extracting DNA? 1.DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2.DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3.DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. 4.DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.Which of the following is true about the denaturation of double-helical DNA? A. Denaturation increases with decreasing temperature. B. Denaturation is accompanied by an increase in the absorption of UV light by DNA. C. G-C rich DNA melts at lower temperature than A-T rich DNA. D. Once denatured, DNA strands cannot anneal.