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- During TLC, what is the stationary phase and what is the mobile phase?Seperation of Amino Acids by thin layer chromotography Lab. Please answer #12 and #13 as I am very confused. Thank you for your help!Which of the following is not a method for measuring reaction rates? A) total pressure B) thin layer chromatography C) isometric barometry D) spectrophotometry E) gas chromatography
- Briefly outline why cinnamaldehyde, cinnamic acid and cinnamyl alcohol travelled different distances on the TLC plate. The solution used to develop the TLC plate was a mixture of toluence and methanol (8:1.5)In TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so slowly?Which of the following bonding interactions are important in the chromatographic separation of ibuprofen on a C18 HPLC column? A. Dispersion forces B. Hydrogen bonding C. Dipole-dipole interactions D. Covalent bonding
- The following are the methods for visualizing spots in TLC chromatogram EXCEPT Choices: Illumination with ultraviolet lamp Using an adsorbent layer that contains a trace of fluorescent dye Treatment of Reagent (e.g. Iodine vapor) None of the aboveThin‑layer chromatography (TLC) can be used as a preliminary identification method for many amphetamine‑type stimulants (ATS). Using a 0.25 mm thick silica gel stationary phase and a 98.5:1.5 methanol:concentrated ammonia mobile phase, a spot was observed to have traveled 3.52 cm when the solvent front traveled 8.81 cm. What is the retardation factor (?F)for the compound that composes this spot?Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explain
- What is chiral chromatography?What would happen to the retention time of a compound if the followingchanges were made?a. Decrease the flow rate of the carrier gasb. Increase the temperature of the columnc. Increase the length of the columnExplain how we would run a paper chromatography on an amino acid sample (explain the procedure or method). In a paper chromatography of an amino acid, the solvent front traveled 12.0 cm while the amino acid traveled 6.0 cm. What is Rf value of the amino acid?