A TLC is developed using a mixture of acetone/methanol (both polar solvents) as mobile phase. The solvent front runs 10 cm. Three spots A, B, and C are spotted with the following distances: A: 3.5 cm B: 8 cm C: 5 cm Which spot is the most polar?
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- The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67 Group of answer choices II > IV > III > I I > III > IV > II I > II > III > IV IV > II > III > IA TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ BThin‑layer chromatography (TLC) can be used as a preliminary identification method for many amphetamine‑type stimulants (ATS). Using a 0.25 mm thick silica gel stationary phase and a 98.5:1.5 methanol:concentrated ammonia mobile phase, a spot was observed to have traveled 3.52 cm when the solvent front traveled 8.81 cm. What is the retardation factor (?F)for the compound that composes this spot?
- The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67An unretained solute is eluted from a GC column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecan (C11H24) is eluted in 17.22 min. What is the retention time (report to 2 decimal places, in minutes) of a compound whose retention index is 1050?4g 5 g 2 g 100 g 49." R Lactic acid Salicylic acid Trichloroacetic acid Flexible collodion qs ad Sig: wart remover. Use as directed. (a) Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 30 g of the mixture? (b) The specific gravity of castor oil is 0.955. How many milliliters of the oil is contained in 30 g of the mixture? (c) If the specific gravity of the mixture is 0.781, what are the percent w/v con- centrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture?
- As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True FalseHow do you calculate the retention factor (Rf) for each spot ? Distance traveled by spot O 5.5cm Distance traveled by spot Y 4.0cm Distance traveled by spot G 3.0cm Distance traveled by solvent front 6.0cmRegarding TLC: a) Compounds A and B are analyzed side-by-side by TLC on silica gel. The developed chromatogram is shown. Which compound is more polar? b) Three unknown mixtures (X, Y, and Z) are analyzed by TLC. The developed plate is shown. What can be concluded about unknown Y? A. Unknown Y contains exactly two different compounds. B. Unknown Y contains at least two different compounds. C. Nothing can be concluded.
- 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.5) Given the data of the diet sodas, which one would you recommend to be the healthiest? (You may need to research the functions of caffeine, aspartame and benzoate. Please add references if necessary.)A new dye (DYE C) was developed and was analyzed against other dyes using paper chromatography. A solution 1:1 ethyl acetate: hexane was used as the eluent. The resulting chromatogram is shown at the photo. Determine the retention factor (Rf) values of each spot on dye C and what are the other dye/s present in dye C.Plant pigments in spinach were separated with TLC with cellulose as thestationary phase and 80/20 diethyl ether/acetone mixture as themobile phase. If needed, the solvent structures in the lab Appendix.1. Determine the Rf values of each plant component on this TLC plate.2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached )