Isolate BO Isolate AIsolate CO Isolate DThe purity and concentration of DNA isolate can be evaluated with the use of UVspectrophotomeleric measurements.measurement of the turbidity of the sample. The organic compounds (containing aromaticAbsorbance reading at 320 provides a generalrings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratiois 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at thiswavelength can be used to estimate the DNA concentration using the equation derived fromBeer's Law:Concentration (pg/mL) = (A260 reading -A320 reading) x 50The absorbance at 280nm is used as an indicator of protein contamination since thearomatic amino acid residues absorb strongly at this wavelengthAnalyze the data given below and determine the following.Isolate AIsolate BIsolate CIsolate DA3200.0510.0910.0650.073A23012271.321.951.44A2604.543.923.884.21A2802.012.112.042.32Question: Which isolate has the ideal 260/230 ratio?

DNA purification is a process used to isolate DNA from other cellular components. The most common…

Answered: Isolate B Isolate A Isolate C Isolate D…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Asolution of double-stranded DNA in 0.2M NaCI gives an A260 of 20 with an extinction coefficient of 20 g'em-'L and a spectrometer path length of lem. What is the concentration oft he solution?
Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.
(MB = methylene blue) What is the concentration of the sample of the substance with the reporter absorbance of 0.40 at 595 nm?
An extraction Buffer, EB(200 mM Trish-HCL(Ph 8), 250 mM NaCl, 25 mM EDTA, 0.5% SDS) is used for DNA extraction from cells. Use their stock solutions to prepare their respective working concentrations for the given working volume of EB. Stock Solutions are: 1M Tris-HCL(pH~8) 0.5M EDTA 0.8M NaCl 10% SDS Show final calculaed values in the table given below. Component 1L EB 500 ml EB 250ml EB 100ml EB 200mM Tris-Hcl 250mM NaCl 25mM EDTA 0.5% SDS dH2O
Estimate the viscosith of 1.0 vol % agarose gel solution if it took 58 minutes for 1500 base pairs long DNA molecule to migrate 11cm during gel electrophoresis under 110V. Assume appropriate numerical values for the missing parameters such as the charge and diameter of DNA molecules.
The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the products were analyzed by electrophoresis on a polyacrylamide. Draw a diagram of the gel banding pattern obtained.
What is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.
Benedict’s Barfoed’s Seliwanoff’s dH2O Negative (-) Negative (-) Negative (-) A Positive (+) Negative (-) Negative (-) B Negative (-) Negative (-) Positive (+) C Positive (+) Positive (+) Negative (-) D Positive (+) Positive (+) Positive (+) honey Positive (+) Positive (+) Strongly Positive (+) X corn syrup Positive (+) Positive (+) Negative (-) Y corn syrup Positive (+) Positive (+) Weakly Positive (+) 1)Explain how you can use the assay results to identify the saccharides in samples A, B, C, and D. Be sure to correlate the structure of each sugar with the chemical feature that each assay tests for. Clearly state the identity of each unknown (A – D). A complete explanation should take at least one long paragraph. Don’t forget to proofread your work to be sure that the explanation is complete and coherent. A. Based on the lab results, what can you conclude about the carbohydrate composition…
ISOLATION AND CHARACTERIZATION OF NUCLEIC ACIDS (DNA) Explain the principle involved in the following chemical tests for the characterization of DNA a. Test for deoxyribose b. Test for phosphate c. Test for purines d. Test for pyrimidines
Can we purify mCherry protein (His-tag attached) with immobilised metal ions affinity chromatography (Ni-IDA)? If so, how can we confirm whether the protein is purified and how to increase its purity?
Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.
Define about sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)? Explain importance of sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ?

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