(MB = methylene blue) What is the concentration of the sample of the substance with the reporter absorbance of 0.40 at 595 nm?

Q: (a) The octapeptide AVGWRVKS was digested with the enzyme trypsin . Which method would be most…

A: Ion-exchange chromatography was the separation technique on the column-based ionic interactions. the…

Q: If you run the Lugol's test on an unknown solution and you get a black color at the end, what does…

A: The use of Lugol's iodine reagent is used to differentiate starch and glycogen from other…

Q: The rate of migration of nucleic acid fragments through an agarose gel is determined by the_

A: The gel electrophoresis is used to separate DNA fragments based on their size, the gel…

Q: Why would a protein would be expected to obtain charge from a charged droplet at a later stage of…

A: The electrospray ionization ( ESI ) technique, help to find the molecular mass of the protein, by…

Q: What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?

A: Biopolymers are polymers, mainly polysaccharides, which are produced by microbes. They are mainly…

Q: Why is R-banding significant when its banding pattern is just the reverse pattern of G-banding?

A: Banding technique: the given banding technique is of chromosome banding techniques i.e. G-banding,…

Q: A student who was isolating aspirin stopped the experiment after the filtration step with alumina.…

A: The melting point is considered as the temperature at which materials changes its form from another…

Q: Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the…

A: Lambert Beer law states that Absorbance of a solution is directly proportional to its concentration…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…

Q: In a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted,  arbitrary)…

A: Size-exclusion chromatography also known as gel filtration chromatography, is a chromatographic…

Q: What does BCR ABL stand for?

A: B-cell receptor (BCR) serve in recognition of the antigens and are present at the surface of the B…

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm…

A: The proteins estimation is a routine process when working with different proteins. The protein…

Q: When combining 0.5 mL of Lysozyme solution (with a concentration of 10 mg/ml) and 0.5 mL of water,…

A: Lysozyme is an enzyme which catalyzes the hydrolysis of peptidoglycans present in cell walls of…

Q: How to graph a scatter plot /X-Y plot of ph and absórbance. pH is the independent variable (x-axis)…

A: pH of tube A400 after 5 minutes           3   0.008     5   0.371     7   0.546     9…

Q: I did an SDS PAGE with BSA as a standard; I noticed in my results that there is a double band - is…

A: SDS-PAGE is Sodium Deodecyl Sulphate Polyacrylamide Gel Electrophoresis. It is a technique that is…

Q: What is alkaline reversion? Explain why this condition gives a false negative result

A: The microbes that affect the intestine of an individual are referred to as enteric pathogens. The…

Q: If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____.…

A: According to the question, If 10 µL of cells in suspension was mixed with 20 µL of a dye called…

Q: what type of gel must be used when the protein size is 2500 Da

A: A type of size exclusion chromatography in which the separation of components is based on the…

Q: What is the use of color reaction tests for proteins in diagnosing diseases? Give an example too!

A: Diagnostics, which can diagnose illness, disorders, or infections, is quickly becoming a critical…

Q: - For a solution in which u = 8.0 X 10 2, calculate K, for %3D

A:

Q: If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to…

A: Biochemistry is the science of study of the internal biochemical processes of organisms. The…

Q: For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t…

A: Answer: Introduction:Thin layer chromatography, or TLC, is an analytical method to analyse mixtures…

Q: When pure hemoglobin (molecular mass of 64,000 Da) is separated by the following methods, what major…

A: Hemoglobin is a tetramer composed of two alpha and two beta subunits. The Molecular weights of the…

Q: Would a sample solution that has a high absorbance reading, have a low or a high concentration

A: Absorbance is a number that measures the diminishing of the transmitted radiant power in a solution.…

Q: Why absorbance is taken at 550nm in Biuret test? Does this absorbance change based on the detection…

A: In the UV-visible area of the spectrum, biological macromolecules including proteins and nucleic…

Q: The GC content of the DNA in the genome of a newly discovered bacterium is 46%, what is the…

A: Chargaff’s rules state that the DNA content from any cell of any organism should always have a 1:1…

Q: Describe how the following technique work on protein purification? ammonium sulfate precipitation

A: Proteins are organic macromolecules that assist in maintaining the structural and functional…

Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.

Q: Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the…

A: Standard curve is made with known concentrations of Bovine serum albumin (BSA) to find the unknown…

Q: What information cannot be obtained from punnet square?

A: The Punnett square is a square used to predict the outcome of a cross or rearing experiment. The…

Q: Why was an SDS-PAGE gel used when working with a protein as opposed to an agarose gel? What dye was…

A: SDS PAGE, abbreviation of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a laboratory…

Q: Is this an drawing of the chemical reaction involved in Lugol’s test?

A: Carbohydrates are molecules that include carbon, hydrogen, and oxygen and have the formula (CH2O)n.…

Q: List two situations when you would not be able to use the UV-Vis absorbance at 280 nm to determine a…

A: There are different ways of determining protein concentration. UV-Vis spectroscopy is one such…

Q: Instead of Molisch's test, what specific test can be used to detect the presence of pentose sugar?…

A: Molisch's test are used for the detection of carbohydrate.

Q: How will you know when the endpoint of the titration is reached? Why is it necessary to mix the…

A: Reaction  It is a process in which reactant molecules react to each other and form another molecule…

Q: How can contaminants in DNA and RNA samples affect absorbance at 260 and concentration results?

A: Introduction: The rings of the bases in nucleic acids (A, T, G, C, and U) are made up of alternating…

Q: Which technique is used to identify an unknown protein ?

A: Proteomics is the study of protein analysis. In this study the comparison, of proteins behaviours…

Q: A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of…

A: Quinine is generally helped to treat malaria that leads by Plasmodium falciparum. This parasite that…

Q: Why is the Mucic Acid test specific for the presence of galactose? Please explain

A: Galactose is a monosaccharide and its molecular formula is same as that of glucose, that is,…

Q: Explain this assertion using the example of hematoxylin and eosin staining.

A: Hematoxylin and eosin staining is used for identification of diseases like cancer. These are two…

Q: What is the expansion of acronym MICA?

A: Immune system includes cells and molecules involved in protecting body against antigens.

Q: He then ran the sample on an agarose gel and observed a strong band at approximately 400bp. What…

A: The band represent a small piece of DNA that was cut with restriction endonuclease and then…

Q: When you graph your protein assay data in Excel, should you include the absorbance of your unknown…

A: Typical protein assays are used to determine protein concentration by comparing the assay response…

Q: What is the driving force behind paper chromatography?

A: Chromatography is a laboratory method of separating the mixture into individual components. It…

Q: What is the basis for the separation of proteins by the following techniques?  (a) gel-filtration…

A: Chromatography is a technique in biophysics that determines three important steps of a mixture of…

Q: For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm…

A: Asked : For same chromatographic column and experiment conditions the answer out of given options is…