ΚΕY: +++ Heavy Agglutination (Gel Solidification) + Medium Agglutination (Thick Liquid) + Slight Agglutination (Flecks) No Reaction Lab Questions:
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- Question: Which base (A, C, T or G) corresponds to X in the unknown? I did the experiment, got the data below, and calculated the binding constants. But I am TOTALLY lost as to how to figure this out! I don't even know what steps I would take. Base pairs Data – all had Temp = 250C PH = 7 Binding Constant A & X [A] = 0.00373221M [X] = 0.00373221M [AX] = 0.0462678M 3.322 C & X [C] = 0.0469007M [X] = 0.0469007M [CX] = 0.00309935M 1.409 T & X [T] = 0.0452279M [X] = 0.0452279M [TX] = 0.00477212M 2.333 G & X [G] = 0.0469554M [X] = 0.0469554M [GX] = 0.00304456M 2.633Two types of ultracentrifugation experiments—sucrose-gradient centrifugation and equilibrium density-gradient centrifugation. Briefly discuss these procedures with respect to the physical bases on which molecular species are separated in each method, the kinds of isotopic compounds used in the analysis, and the biological processes that have been or can be analyzed with each.What are the reaction bat the molecular level of denaturation of frying egg. Explain step by step procedure in an informative and objective way.
- Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280 nm. The sample was diluted (200 μL of purified antibody in 800 μL buffer) prior to analysis using a spectrophotometer. Calculate the concentration of antibody in the purified fraction if the Abs=0.95 of the diluted antibody. The molar absorptivity is known to be 191,411.6 M-1cm-1 and the molecular weight is 150 kDa. The pathlength for the cuvette is 1 cm.THOUGHT QUESTION Imagine we identify a gene that is directly responsible for the effects of vasopressin on male mammals, including humans-we will call it trust1-that leads to the production of a vasopressin receptor in the brain, which we will call TRUST1. There are different versions of trust1, all of which lead to different levels of the behavior associated with this neuropeptide on male behavior. Give some examples where it would be a good idea to know a particular males genotype-that is, which of the trust1 genes he has. Give an example of when you think science has gone too far and this information should not be known.Experiment: Bradford protein assaygive the answers (4-5 lines) of review questions in the end. answer should be logical and understandable and without plagiarism. avoid copy pasting. Background information:The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assessing protein concentrations for gel electrophoresis. The method described below is for a 100 µl sample volume using 5 ml color reagent. It is sensitive to about 5 to 200 micrograms protein, depending on the dye quality. In assays using 5 ml color reagent prepared in lab, the sensitive range is closer to 5 to 100 µg protein. Scale down the volume for the "micro assay procedure," which uses 1 ml cuvettes. Protocols, including use of microliter plates are described in the flyer that comes with…
- After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.General Biology - Help me do this activity, you can read the instructions for clarification, thanks!paper chromatography: 1.If the Rf of blue was 0.81, Rf of yellow was 0.68, and Rf of red was 0.64, then which color would be the heaviest? which one would be the lightest? and why? Gel electrophoresis: 2.Given that well 1 represented protein from a dog (Rf=0.45), well 2 from a wolf (Rf=0.73) and well 3 from a fox (Rf=0.76), and the unknown well represented protein from a "chupacabra". what animal would say the "chupacabra" is closely related to? 3.which one would be the heaviest and lightest in given above electrophoresis? and why?
- The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?All questions a) What is the hypothesis of Griffith’s experiment? b) Why did mouse No. 3 survived? c) Why did mouse No. 4 died? d) If Griffith had injected a fifth mouse with a combination of heat-killed R strain bacteria and heat-killed S strain bacteria, would the mouse have died? Give your reason.Hey I am practicing for a molecular biology test and I am having trouble with is practice problem. I am not sure how to set it up to determine the amount of Acid Phosphatase. Can you help? Determine the amount of Acid Phosphatase in micrograms/ gram wheat germ from the following experimental values. The amount of acid phosphatase found in 198 ul of extract was 8 ug. The amount of extraction buffer used was 8 mL and the amount of wheat germ was 0.8 g.
