Please pick among these 4 Protein chromatography is typically column-based: — lon-exchange — Reverse-phase/Hydrophobic interaction — Gel-filtration — Affinity
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Please pick among these 4
Protein chromatography is typically column-based:
— lon-exchange
— Reverse-phase/Hydrophobic interaction
— Gel-filtration
— Affinity
Step by step
Solved in 3 steps
- HbA1c is used to monitor blood glucose levels because hemoglobin is the only protein in blood that is covalently modified by glucose. True False Insulin Glargine is a long-acting form of insulin that is synthesized with several D-amino acids that slow its proteolytic degradation and extend the half-life of the insulin Glargine molecule. True FalseDifferentiate between pro-insulin and mature insulin. Name the commonly used vector for cloning genes into higher organisms.You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not morethan 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for thefollowing questions?1) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide genefrom the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 whichhas a 5-recognition site and a 3- HindIII in the MCS?
- You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not morethan 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for thefollowing questions? ) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5-recognition site and a 3- HindIII in the MCS?You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during absorptive state. q: Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide genefrom the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 whichhas a 5-recognition site and a 3`- HindIII in the MCS?You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during absorptive state. q1 : How can you guarantee a high expression of your protein in any expression vector? answer please
- You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during absorptive state. provide answers for thefollowing questions?1) Which tube from the three is the most appropriate to use and why? 2) How can you guarantee a high expression of your protein in any expression vector?You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more than 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for the following questions? ( please answer all part 1, 2 and 3 ) 1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5′-recognition site and a 3′- HindIII in the MCS? 3) How…You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. 1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5¢-recognition site and a 3¢- HindIII in the MCS? 3) How can you guarantee a high expression of your protein in any expression vector?
- you are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more than 2 pages provide answers for the following question? Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5¢-recognition site and a 3¢- HindIII in the MCS?You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not morethan 2 pages provide answers for the following questions? 1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5-recognition site and a 3- HindIII in the MCS? 3) How can you guarantee a high expression of your protein in any expression vector?explain the following prperties of G protein: structure of G- activation cycle and signaling pathway for Gaq